AFM and MFM techniques for enzyme activity imaging and quantification

被引：7

作者：

Arredondo, Michelle ^{[1
,2
]}

Stoytcheva, Margarita ^{[2
]}

Morales-Reyes, Israel ^{[3
]}

Batina, Nikola ^{[3
]}

机构：

[1] Tecnol Nacl Mexico, Inst Tecnol Mexicali, Mexicali, Baja California, Mexico

[2] Univ Autonoma Baja California, Inst Ingn, Mexicali, Baja California, Mexico

[3] Univ Autonoma Metropolitana Iztapalapa, Dept Quim, Div Ciencias Basicas & Ingn, Lab Nanotecnol & Ingn Mol, Mexico City, DF, Mexico

来源：

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT | 2018年 / 32卷 / 04期

关键词：

Atomic force microscopy; magnetic force microscopy; trypsin activity; ferrogel; ATOMIC-FORCE MICROSCOPY; TAPPING MODE; MOLECULES; IMMOBILIZATION; SPECTROSCOPY; REACTIVATORS; HYDROLYSIS; INHIBITORS; SECM;

D O I：

10.1080/13102818.2018.1470904

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this work, a new approach for enzyme activity monitoring is suggested. It is based on the real-time imaging, by atomic force microscopy (AFM) and magnetic force microscopy (MFM), of the degradation of a nanoparticles-loaded enzyme responsive layer, namely Fe3O4-nanoparticlesloaded gelatin. The roughness analysis allowed the quantification of the trypsin-induced ferrogel degradation and the correlation of the AFM and MFM data obtained.

引用

页码：1065 / 1074

页数：10

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