Analysis of the Bombyx mori nucleopolyhedrovirus ie-1 promoter in insect, mammalian, plant, and bacterial cells

被引：7

作者：

Fujita, Ryosuke ^{[1
,2
]}

Ono, Chikako ^{[1
,3
]}

Ono, Isamu ^{[1
]}

Asano, Shin-ichiro ^{[1
]}

Bando, Hisanori ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Agr, Div Appl Biosci, Lab Appl Mol Entomol, Sapporo, Hokkaido 0608589, Japan

[2] Natl Inst Infect Dis, Dept Med Entomol, Tokyo 1628640, Japan

[3] Osaka Univ, Dept Mol Virol, Microbial Dis Res Inst, Osaka 5650871, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2015年 / 464卷 / 04期

关键词：

Baculovirus; Promoter; Insect; Host range; Bombyx mori nucleopolyhedrovirus; NUCLEAR POLYHEDROSIS-VIRUS; MULTIPLE NUCLEOPOLYHEDROVIRUS; SEQUENCE-ANALYSIS; ACTIN GENE; EXPRESSION; TRANSCRIPTION; GENOME; IDENTIFICATION; VECTOR; REPLICATION;

D O I：

10.1016/j.bbrc.2015.07.126

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Bombyx mori nucleopolyhedrovirus (BmNPV) ie-1 promoter exhibits strong transcriptional activity and is used in transient foreign gene expression systems in insect cells. In a reporter assay experiment using the BmNPV ie-1 promoter, we found that it exhibited activity even in non-host mammalian BHK cells, plant BY-2 cells, and also bacterial Escherichia coli cells. An analysis using a deletion series of the BmNPV ie-1 promoter demonstrated that the core promoter region of this promoter was sufficient to display promoter activity in BHK cells, BY-2 cells, and E. coli cells, whereas upstream elements were required for higher activity in insect cells. Furthermore, we found that the BmNPV ie-1 promoter exhibited sufficient activity for a beta-galactosidase assay in E. coil cells. The results obtained here suggest that the BmNPV ie-1 promoter has potential as a universal promoter for transient expression systems in insect, mammalian, plant, and bacterial cells. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：1297 / 1301

页数：5

共 31 条

[1] The sequence of the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus genome [J].