Evaluation of the anti-inflammatory effects of selected cannabinoids and terpenes from Cannabis Sativa employing human primary leukocytes

被引：20
作者：
Blevins, Lance K. [1 ]
Bach, Anthony P. [1 ]
Crawford, Robert B. [1 ]
Zhou, Jiajun [1 ,2 ]
Henriquez, Joseph E. [1 ,3 ]
Rizzo, Michael D. [1 ,4 ]
Sermet, Sera [1 ,3 ]
Khan, D. M. Isha Olive [1 ,3 ]
Turner, Helen [6 ]
Small-Howard, Andrea L. [7 ]
Kaminski, Norbert E. [1 ,3 ,5 ]
机构：
[1] Michigan State Univ, Inst Integrat Toxicol, E Lansing, MI 48824 USA
[2] Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI USA
[3] Michigan State Univ, Dept Pharmacol & Toxicol, E Lansing, MI USA
[4] Michigan State Univ, Cell & Mol Biol Program, E Lansing, MI USA
[5] Michigan State Univ, Ctr Res Ingredient Safety, E Lansing, MI USA
[6] Chaminade Univ Honolulu, Lab Immunol & Signal Transduct, Honolulu, HI USA
[7] GbS Global Biopharm, Ottawa, ON, Canada
来源：
FOOD AND CHEMICAL TOXICOLOGY | 2022年 / 170卷
关键词：
Cannabinoids; Cannabis; Cannabidiol; Terpenes; Anti-inflammatory; Immunomodulation; EQUILIBRATIVE NUCLEOSIDE TRANSPORTER; RECEPTOR; EXPRESSION; INFLAMMATION; MONOCYTES; GROWTH; CELLS; MODEL;
D O I：
10.1016/j.fct.2022.113458
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Cannabis is well established as possessing immune modulating activity. The objective of this study was to evaluate the anti-inflammatory properties of selected cannabis-derived terpenes and cannabinoids. Based on their activity in cannabis-chemovar studies, alpha-pinene, trans-nerolidol, D-limonene, linalool and phytol were the selected terpenes evaluated. The cannabinoid compounds evaluated included cannabidivarin, cannabidiol, cannabinol, cannabichromene, cannabigerol and delta-9-tetrahydrocannabinol. Human PBMC were pretreated with each compound, individually, at concentrations extending from 0.001 to 10 mu M and then stimulated with CpG (plasmacytoid dendritic cell), LPS (monocytes), or anti-CD3/CD28 (T cells). Proliferation, activation marker expression, cytokine production and phagocytosis, were quantified. Of the 21 responses assayed for each com-pound, cannabinoids showed the greatest immune modulating activity compared to their vehicle control. Delta-9-tetrahydrocannabinol possessed the greatest activity affecting 11 immune parameters followed by cannabi-divarin, cannabigerol, cannabichromene, cannabinol and cannabidiol. alpha-Pinene showed the greatest immune modulating activity from the selected group of terpenes, followed by linalool, phytol, trans-nerolidol. Limonene had no effect on any of the parameters tested. Overall, these studies suggest that selected cannabis-derived terpenes displayed minimal immunological activity, while cannabinoids exhibited a broader range of activity. Compounds possessing anti-inflammatory effects may be useful in decreasing inflammation associated with a range of disorders, including neurodegenerative disorders.
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页数：13
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