Heme oxygenase-1 promotes Caco-2 cell proliferation and migration by targeting CTNND1

被引：15
作者：
Zhang Li [1 ]
Liu Yu-lin [1 ]
Chen Guang-xiang [1 ]
Cui Bin [1 ]
Wang Jin-shen [1 ]
Shi Yu-long [1 ]
Li Le-ping [1 ]
Guo Xiao-bo [1 ]
机构：
[1] Shandong Univ, Prov Hosp, Dept Gastrointestinal Surg, Jinan 250021, Shandong, Peoples R China
来源：
CHINESE MEDICAL JOURNAL | 2013年 / 126卷 / 16期
关键词：
heme oxygenase-1; Caco-2; cells; microarray; CTNND1; ISCHEMIA/REPERFUSION INJURY; REPERFUSION INJURY; COLD PRESERVATION; LUNG INJURY; RAT LIVERS; P120-CATENIN; PROTECTS; ISCHEMIA; OVEREXPRESSION; INHIBITION;
D O I：
10.3760/cma.j.issn.0366-6999.20130196
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Background Heme oxygenase-1 (HO-1) can be induced by inflammatory cytokines, oxidation, ischemia, hypoxia, and endotoxins. As a "graft survival protective gene," HO-1 is a hot spot in organ transplantation research. However, the role of HO-1 gene expression in the function of human colon adenocarcinoma cell line (Caco-2) cells has not been reported previously. Methods The role of HO-1 in the proliferation and migration of Caco-2 cells was analyzed using a stable HO-1 expression plasmid. We constructed a recombinant adeno-associated virus plasmid containing the HO-1 gene, heme oxygenase 1 (HMOX1), which was transfected into Caco-2 intestinal cells. We identified a number of target genes by global microarray analysis combined with real-time polymerase chain reaction (PCR) and chromatin immunoprecipitation assay. Results Our results showed that significant HO-1 upregulation was demonstrated in the Caco-2 cells after HO-1 transfection. Restoration of HO-1 expression promoted proliferation and invasion in vitro. The CTNND1 gene, a member of the armadillo protein family, was identified as a direct HO-1 target gene. Conclusion Overexpression of HO-1 promotes Caco-2 cell proliferation and migration by targeting the CTNND1 gene.
引用
收藏
页码：3057 / 3063
页数：7
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