Characterization and localization to chromosome 7 of ψhGABPα, a human processed pseudogene related to the ets transcription factor, hGABPα

GABP is a heteromeric transcription factor complex which consists of the ets related protein, GABP alpha, and the Notch-related protein, GABP beta. We isolated a human genomic DNA fragment which is highly homologous and colinear with human GABPa cDNA, but which lacks introns. This processed pseudogene, psi hGABP alpha, is expressed as RNA in U937 human myeloid cells, but a mutation at the site that corresponds to the ATC start methionine codon prevents its translation into protein. The pseudogene was localized to chromosome 7 using a somatic cell hybrid mapping panel and it is not syntenic with authentic GABPa, which was localized to chromosome 21. We have identified psi hGABP alpha, a novel, GABP alpha-related processed pseudogene which is expressed as a RNA transcript in human myeloid cells. (C) 1999 Published by Elsevier Science B.V. All rights reserved.