A mutation inAsparagine-Linked Glycosylation 12(ALG12) leads to receptor misglycosylation and attenuated responses to multiple microbial elicitors

被引：4

作者：

Trempel, Fabian ^{[1
]}

Eschen-Lippold, Lennart ^{[1
]}

Bauer, Nicole ^{[1
]}

Ranf, Stefanie ^{[1
,2
]}

Westphal, Lore ^{[1
]}

Scheel, Dierk ^{[1
]}

Lee, Justin ^{[1
]}

机构：

[1] Leibniz Inst Plant Biochem, Weinberg 3, D-06120 Halle, Germany

[2] Tech Univ Munich, TUM Sch Life Sci Weihenstephan, Freising Weihenstephan, Germany

来源：

FEBS LETTERS | 2020年 / 594卷 / 15期

关键词：

asparagine-linked glycosylation; calcium; membrane receptors; signalling; NADPH OXIDASE RBOHD; PATTERN-RECOGNITION RECEPTORS; BRASSINOSTEROID RECEPTORS; ENDOPLASMIC-RETICULUM; DEFENSE RESPONSES; INNATE IMMUNITY; KINASE BIK1; ARABIDOPSIS; GLYCOSYLATION; SIGNAL;

D O I：

10.1002/1873-3468.13850

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Changes in cellular calcium levels are one of the earliest signalling events in plants exposed to pathogens or other exogenous factors. In a genetic screen, we identified anArabidopsis thaliana'changed calcium elevation 1' (cce1) mutant with attenuated calcium response to the bacterial flagellin flg22 peptide and several other elicitors. Whole-genome resequencing revealed a mutation inasparagine-linked glycosylation 12that encodes the mannosyltransferase responsible for adding the eighth mannose residue in an alpha-1,6 linkage to the dolichol-PP-oligosaccharideN-glycosylation glycan tree precursors. While properly targeted to the plasma membrane, misglycosylation of several receptors in thecce1background suggests thatN-glycosylation is required for proper functioning of client proteins.

引用

页码：2440 / 2451

页数：12

共 49 条

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