Scopoletin:: An inducible nitric oxide synthesis inhibitory active constituent from Artemisia feddei

被引：57

作者：

Kang, TH

Pae, HO

Jeong, SJ

Yoo, JC

Choi, BM

Jun, CD

Chung, HT

Miyamoto, T

Higuchi, R

Kim, YC ^{[1
]}

机构：

[1] Wonkwang Univ, Coll Pharm, Iksan 570749, Chonbuk, South Korea

[2] Wonkwang Univ, Sch Med, Iksan 570749, Chonbuk, South Korea

[3] Wonkwang Univ, Med Resources Res Ctr, Iksan 570749, Chonbuk, South Korea

[4] Kyushu Univ, Fac Pharmaceut Sci, Fukuoka 812, Japan

来源：

PLANTA MEDICA | 1999年 / 65卷 / 05期

关键词：

Artemisia feddei; Compositae; scopoletin; nitric oxide; inducible nitric oxide synthesis;

D O I：

10.1055/s-1999-14014

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Bioassay-guided fractionation of an H2O extract of Artemisia feddei has furnished an inducible nitric oxide synthase (iNOS) inhibitory coumarin, scopoletin (1) and one of the inactive sesquiterpenes, achillin (2). Compound 1 showed inhibition of nitric oxide (NO) synthesis in a dose-dependent manner in murine macrophage-like RAW 264.7 cells stimulated with interferon-gamma (IFN-gamma) plus lipopolysaccharide (LPS). The inhibition of NO synthesis of 1 was due to suppression of iNOS mRNA and iNOS protein, as determined by Northern and Western blotting, respectively.

引用

页码：400 / 403

页数：4

共 14 条

[1] AUFFRAY C, 1980, EUR J BIOCHEM, V107, P303
[2] Inducible nitric oxide synthase and proinflammatory cytokine expression by human keratinocytes during acute urticaria
Becherel, PA
Chosidow, O
LeGoff, L
Frances, C
Debre, P
Mossalayi, MD
Arock, M
[J]. MOLECULAR MEDICINE, 1997, 3 (10) : 686 - 694
[3] Nitric oxide inhalation transiently elevates pulmonary levels of cGMP, iNOS mRNA, and TNF-α
Brady, TC
Crapo, JD
Mercer, RR
[J]. AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 1998, 275 (03) : L509 - L515
[4] CLANCY RM, 1995, P SOC EXP BIOL MED, V210, P93
[5] The Griess assay: Suitable for a bio-guided fractionation of anti-inflammatory plant extracts?
Dirsch, VM
Stuppner, H
Vollmar, AM
[J]. PLANTA MEDICA, 1998, 64 (05) : 423 - 426
[6] JUN CD, 1994, J IMMUNOL, V153, P3684
[7] LEE TB, 1989, ILLUSTRATED FLORA KO, P758
[8] MANTHEY CL, 1994, J IMMUNOL, V152, P825
[9] STUDIES ON SESQUITERPENELACTONES .11. CHEMICAL CONSTITUTION OF ARTEMISIA-FEDDEI LEV ET VAN
MATSUEDA, S
NAGAKI, M
KOREEDA, M
[J]. YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 1980, 100 (06): : 615 - 618
[10] MINEO M, 1997, BIOCHEM BIOPH RES CO, V239, P367

← 1 2 →