Differentiating Ovine BSE from CH1641 Scrapie by Serial Protein Misfolding Cyclic Amplification

被引：8
作者：
Taema, Maged M. [1 ]
Maddison, Ben C. [2 ]
Thorne, Leigh [3 ]
Bishop, Keith [2 ]
Owen, Jonathan [2 ]
Hunter, Nora [4 ]
Baker, Claire A. [2 ]
Terry, Linda A. [3 ]
Gough, Kevin C. [1 ]
机构：
[1] Univ Nottingham, Sch Vet Med & Sci, Loughborough LE12 5RD, Leics, England
[2] Univ Nottingham, Sch Vet Med & Sci, ADAS UK, Loughborough LE12 5RD, Leics, England
[3] Anim Hlth & Vet Labs Agcy, Addlestone KT15 3NB, Surrey, England
[4] Univ Edinburgh, Roslin Inst, Neuropathogenesis Div, Easter Bush EH25 9RG, Midlothian, Scotland
来源：
MOLECULAR BIOTECHNOLOGY | 2012年 / 51卷 / 03期
基金：
英国生物技术与生命科学研究理事会;
关键词：
Scrapie; Prion; Strain; BSE; CH1641; BOVINE SPONGIFORM ENCEPHALOPATHY; IN-VITRO AMPLIFICATION; PRION PROTEIN; MOLECULAR ANALYSIS; SHEEP BREEDS; NATURAL SCRAPIE; ITALIAN SHEEP; DISEASE; BRAIN; GENOTYPE;
D O I：
10.1007/s12033-011-9460-0
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Whilst ovine BSE displays distinct pathological characteristics to ovine CH1641-like scrapie upon passage in rodents, they have very similar molecular phenotypes. As such, the in vitro differentiation of these strains in routine surveillance programmes presents a significant diagnostic challenge. In this study, using serial protein-misfolding cyclic amplification (sPMCA), ovine BSE was readily amplified in vitro in brain substrates from sheep with V(136)R(154)Q(171)/V(136)R(154)Q(171) or AHQ/AHQ PRNP genotypes. In contrast, the CH1641 strain was refractory to such amplification. This method allowed for complete and unequivocal differentiation of experimental BSE from CH1641 prion strains within an ovine host.
引用
收藏
页码：233 / 239
页数：7
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