Differentiation of fetal pancreatic stem cells into neuron-like and islet-like cells in vitro

被引：1

作者：

Hua, Xiufeng ^{[1
]}

Wang, Yanwei ^{[2
]}

Lian, Peiwen ^{[2
]}

Zhang, Shouxin ^{[2
]}

Li, Jianyuan ^{[2
]}

Wang, Haiyan ^{[2
]}

Chen, Shulin ^{[1
]}

Gao, Wei ^{[1
]}

机构：

[1] Yuhuangding Hosp, Dept Endocrinol, Yantai 264000, Shandong, Peoples R China

[2] Yuhuangding Hosp, Cent Lab, Res Ctr Stem Cell Engn Shandong, Yantai 264000, Shandong, Peoples R China

来源：

NEURAL REGENERATION RESEARCH | 2012年 / 7卷 / 07期

关键词：

fetal pancreas; pancreatic stem cells; differentiation; islet-like cells; neuron-like cells; neural regeneration; PROGENITOR CELLS; ADULT-MOUSE; INSULIN; PHENOTYPES; EGF; LINEAGES; GASTRIN; GROWTH; MICE;

D O I：

10.3969/j.issn.1673-5374.2012.07.005

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Pancreatic stem cells were isolated and cultured from aborted human fetal pancreases of gestational age 14-20 weeks. They were seeded at a density of 1 x 10(4) in serum-free media for differentiation into neuron-like cells, expressing beta-tubulin III and glial fibrillary acidic protein. These neuron-like cells displayed a synapse-like morphology and appeared to form a neuronal network. Pancreatic stem cells were also seeded at a density of 1 x 10(5) for differentiation into islet-like cells, expressing insulin and glucagon, with an islet-like morphology. These cells had glucose-stimulated secretion of human insulin and C-peptide. Results suggest that pancreatic stem cells can be differentiated into neuron-like and islet-like cells.

引用

页码：506 / 510

页数：5

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