Pathogenicity in domestic ducks and mice of highly pathogenic H5N1 clade 2.3.2.1 influenza viruses recently circulating in Eastern Asia

被引:7
作者
Kang, Hyun-Mi [1 ]
Choi, Jun-Gu [1 ]
Kim, Kwang-Il [1 ]
Kim, Bang-Sil [1 ]
Batchuluun, Damdinjav [2 ]
Erdene-Ochir, Tseren-Ochir [2 ]
Kim, Min-Chul [1 ]
Kwon, Jun-Hun [1 ]
Park, Choi-Kyu [3 ]
Lee, Youn-Jeong [1 ]
机构
[1] Anim & Plant Quarantine Agcy, Avian Dis Div, Anyangsi 430757, Gyeonggido, South Korea
[2] State Cent Vet Lab, Transboundary Anim Dis Lab, Avian Influenza Sect, Ulaanbaatar 210153, Mongolia
[3] Kyungpook Natl Univ, Coll Vet Med, Taegu 702701, South Korea
关键词
H5N1; HPAI; South Korea; Mongolia; Domestic duck; Mice; AVIAN INFLUENZA; WILD BIRDS; HONG-KONG; A VIRUSES; VIRULENCE; EVOLUTION; POULTRY;
D O I
10.1016/j.vetmic.2013.09.013
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Influenza virus A (H5N1) clade 2.3.2.1 has recently caused widespread outbreaks of disease in domestic poultry and wild birds in Eastern Asia. In the current study, the antigenicity and pathogenicity of three clade 2.3.2.1 viruses (Ck/Kr/Gimje/08, Ws/Mongolia/1/09, and Ws/Mongolia/7/10) were investigated in domestic ducks and mice. The H5N1 influenza viruses in this study were antigenically similar to each other (r-values of 0.35-1.4). The three viruses replicated systemically in all tissues tested in domestic ducks, indicating high pathogenicity. However, the viruses produced different clinical signs and mortality rates: Ck/Kr/Gimje/08 and Ws/Mongolia/1/09 resulted in 100% mortality with severe neurological signs, whereas Ws/Mongolia/7/10 resulted in 50% mortality with relatively mild neurological signs. In mice, infection with Ck/Kr/Gimje/08 and Ws/Mongolia/7/10 resulted in weight loss that peaked at 4 days post-infection (22.3% and 20.8%, respectively), same MLD50 (2.2 Log(10) EID50) and systemic replication. The three viruses had K deletion at the 2 position of the HA1-connecting peptide (PQRERRRK-R), which is associated with increased virulence in domestic ducks and harbored NA stalk deletion, NS1 deletion and mutation of P42S in NS1, and full length (90aa) in PB1-F2, which confer increased virulence in mice. Our study shows that clade 2.3.2.1 viruses from Korea and Mongolia are antigenically similar and highly pathogenic in both domestic ducks and mice. Moreover, we provide molecular determinants of the clade 2.3.2.1 viruses associated with the pathogenicity in domestic ducks and mice, respectively. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:327 / 333
页数:7
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