On-line post-column photochemical derivatization in liquid chromatographic-diode-array detection analysis of binary drug mixtures

被引:44
作者
DiPietra, AM [1 ]
Andrisano, V [1 ]
Gotti, R [1 ]
Cavrini, V [1 ]
机构
[1] UNIV BOLOGNA,DIPARTIMENTO SCI FARMACEUT,I-40126 BOLOGNA,ITALY
关键词
diode-array detection; drug analysis; high-performance liquid chromatography; pharmaceutical creams; photochemical derivatization; solid-phase extraction;
D O I
10.1016/0731-7085(95)01695-3
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
HPLC methods were developed for the analysis of pharmaceutical creams containing binary drug mixtures (betamethasone valerate-chlorocresol; hydrocortisone-miconazole nitrate; desonide pivalate-chlorhexidine; dexamethasone-clolrimazole; triamcinolone acetonide-econazole nitrate). The chromatographic separations were performed on C-18 and cyano columns under reversed-phase conditions. A post-column on-line photochemical reactor (irradiation at 254 nm) was arranged between the analytical column and the diode-array detector to enhance the performance of the method. Two UV spectra (photoreactor on and off) were obtained for each analyte and these additional sources of information proved to be useful for the unambiguous identification of the various analytes. The method was applied to the quality control of commercial creams using a solid-phase extraction procedure for the sample clean-up.
引用
收藏
页码:1191 / 1199
页数:9
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