Synthesis of selectively 15N- or 13C-labelled malachite green

被引：6

作者：

DeFina, SC ^{[1
]}

Dieckmann, T ^{[1
]}

机构：

[1] Univ Calif Davis, Dept Chem, Davis, CA 95616 USA

来源：

JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS | 2002年 / 45卷 / 03期

关键词：

N-15-; C-13; aptamer; malachite green; NMR spectroscopy;

D O I：

10.1002/jlcr.554

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two complimentary syntheses of selectively N-15- or C-13-labelled malachite green hydrochloride were developed in order to provide labelled ligands for structural studies of RNA aptamer/ligand complexes. The N-15- and C-13- labelled versions of the dye have been used in NMR studies to probe the changes in the electronic charge distribution and dynamics of the dye upon binding to RNA. Copyright (C) 2002 John Wiley Sons, Ltd.

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页码：241 / 248

页数：8

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