Constituting a glutathione S-transferase-cocktail vaccine against tick infestation

被引：27
作者：
Ndawula Jr, Charles [1 ]
Sabadin, Gabriela Alves [1 ]
Parizi, Luis Fernando [1 ]
Vaz Jr, Itabajara da Silva [1 ,2 ,3 ]
机构：
[1] Univ Fed Rio Grande do Sul, Ctr Biotecnol, Ave Bento Goncalves 9500, BR-91501970 Porto Alegre, RS, Brazil
[2] Univ Fed Rio Grande do Sul, Fac Vet, Ave Bento Goncalves 9090, BR-91540000 Porto Alegre, RS, Brazil
[3] Inst Nacl Ciencia & Tecnol Entomol Mol, Ave Bento Goncalves 9090, BR-91501970 Porto Alegre, RS, Brazil
来源：
VACCINE | 2019年 / 37卷 / 14期
关键词：
GST; Rhipicephalus appendiculatus; African ticks; Broad-spectrum vaccine; RHIPICEPHALUS BOOPHILUS MICROPLUS; HAEMAPHYSALIS-LONGICORNIS; CANDIDATE ANTIGENS; ANTIBODY AVIDITY; CATTLE; EXPRESSION; STRATEGIES; PROTEINS; DIFFERENTIATION; APPENDICULATUS;
D O I：
10.1016/j.vaccine.2019.02.039
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Cocktail vaccines are proposed as an attractive way to increase protection efficacy against specific tick species. Furthermore, such vaccines made with different tick antigens have the potential of cross-protecting against a broad range of tick species. However, there are still limitations to the selection of immunogen candidates. Acknowledging that glutathione S-transferases (GSTs) have been exploited as vaccines against ticks and other parasites, this study aimed to analyze a GST-cocktail vaccine as a potential broad-spectrum tick vaccine. To constitute the GST-cocktail vaccine, five tick species of economic importance for livestock industry were studied (Rhipicephalus appendiculatus, Rhipicephalus decoloratus, Rhipicephalus microplus, Amblyomma variegatum, and Haemaphysalis longicornis). Tick GST ORF sequences were cloned, and the recombinant GSTs were produced in Escherichia coli. rGSTs were purified and inoculated into rabbits, and the immunological response was characterized. The humoral response against rGST-Rd and rGST-Av showed a stronger cross-reactivity against heterologous rGSTs compared to rGST-HI, rGST-Ra, and rGST-Rm. Therefore, rGST-Rd and rGST-Av were selected for constituting an experimental rGST-cocktail vaccine. Vaccination experiment in rabbits showed that rGST-cocktail caused 35% reduction in female numbers in a Rhipicephalus sanguineus infestation. This study brings forward an approach to selecting immunogens for cocktail vaccines, and the results highlight rGST-Rd and rGST-Av as potentially useful tools for the development of a broad-spectrum tick vaccine. (C) 2019 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1918 / 1927
页数：10
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