MS-Based Monitoring of Proteolytic Decay of Synthetic Reporter Peptides for Quality Control of Plasma and Serum Specimens

被引：12

作者：

Findeisen, Peter ^{[1
]}

Thumfart, Joerg Oliver ^{[1
]}

Costina, Victor ^{[1
]}

Hofheinz, Ralf ^{[2
]}

Neumaier, Michael ^{[1
]}

机构：

[1] Heidelberg Univ, Inst Clin Chem, Med Fac Mannheim, Univ Hosp Mannheim, D-68167 Mannheim, Germany

[2] Heidelberg Univ, Med Clin 3, Med Fac Mannheim, Univ Hosp Mannheim, D-68167 Mannheim, Germany

来源：

AMERICAN JOURNAL OF CLINICAL PATHOLOGY | 2013年 / 140卷 / 03期

关键词：

Biobanking; Quality assessment; Serum; Plasma; Protease; Reporter peptides; Decay; Mass spectrometry; ASSISTED-LASER-DESORPTION/IONIZATION; BIOMARKER DISCOVERY; PROTEOME; TIME; SAMPLES; VALIDATION; DIAGNOSIS; PROTEINS; IMPACT; CANCER;

D O I：

10.1309/AJCPOS9Z5KVZSFSC

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Objectives: To determine the preanalytical quality of serum and plasma by monitoring the time-dependent ex vivo decay of a synthetic reporter peptide (RP) with liquid chromatography/mass spectrometry (LC/MS). Methods: Serum and plasma specimens were spiked with the RP and proteolytic fragments were monitored with LC/MS at different preanalytical time points ranging from 2 to 24 hours after blood withdrawal. Results: The concentration of fragments changed in a time-dependent manner, and respective peptide profiles were used to classify specimens according to their preanalytical time span. Classification accuracy was high, with values always above 0.89 for areas under receiver operating characteristic curves. Conclusions: This "proteomics degradation clock" can be used to estimate the preanalytical quality of serum and plasma and might have impact on quality control procedures of biobanking repositories.

引用

收藏

页码：314 / 323

页数：10

相关论文

共 33 条

[11] High-abundance polypeptides of the human plasma proteome comprising the top 4 logs of polypeptide abundance [J].

Hortin, Glen L. ;

Sviridov, Denis ;

Anderson, N. Leigh .

CLINICAL CHEMISTRY, 2008, 54 (10) :1608-1616

[12] Analytical and preanalytical biases in serum proteomic pattern analysis for breast cancer diagnosis [J].

Karsan, A ;

Eigl, BJ ;

Flibotte, S ;

Gelmon, K ;

Switzer, P ;

Hassell, P ;

Harrison, D ;

Law, J ;

Hayes, M ;

Stillwell, M ;

Xiao, Z ;

Conrads, TP ;

Veenstra, T .

CLINICAL CHEMISTRY, 2005, 51 (08) :1525-1528

[13] Preanalytical variability: the dark side of the moon in laboratory testing [J].

Lippi, G ;

Guidi, GC ;

Mattiuzzi, C ;

Plebani, M .

CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2006, 44 (04) :358-365

[14] The application of atmospheric pressure matrix-assisted laser desorption/ionization to the analysis of long-term cryopreserved serum peptidome [J].

Mangerini, Rosa ;

Romano, Paolo ;

Facchiano, Angelo ;

Damonte, Gianluca ;

Muselli, Marco ;

Rocco, Mattia ;

Boccardo, Francesco ;

Profumo, Aldo .

ANALYTICAL BIOCHEMISTRY, 2011, 417 (02) :174-181

[15] SELDI-TOF MS whole serum proteomic profiling with IMAC surface does not reliably detect prostate cancer [J].

McLerran, Dale ;

Grizzle, William E. ;

Feng, Ziding ;

Thompson, Ian M. ;

Bigbee, William L. ;

Cazares, Lisa H. ;

Chan, Daniel W. ;

Dahlgren, Jackie ;

Diaz, Jose ;

Kagan, Jacob ;

Lin, Daniel W. ;

Malik, Gunjan ;

Oelschlager, Denise ;

Partin, Alan ;

Randolph, Timothy W. ;

Sokoll, Lori ;

Srivastava, Shiv ;

Srivastava, Sudhir ;

Thornquist, Mark ;

Troyer, Dean ;

Wright, George L. ;

Zhang, Zhen ;

Zhu, Liu ;

Semmes, O. John .

CLINICAL CHEMISTRY, 2008, 54 (01) :53-60

[16] Analytical validation of serum proteomic profiling for diagnosis of prostate cancer: Sources of sample bias [J].

McLerran, Dale ;

Grizzle, William E. ;

Feng, Ziding ;

Bigbee, William L. ;

Banez, Lionel L. ;

Cazares, Lisa H. ;

Chan, Daniel W. ;

Diaz, Jose ;

Izbicka, Elzbieta ;

Kagan, Jacob ;

Malehorn, David E. ;

Malik, Gunjan ;

Oelschlager, Denise ;

Partin, Alan ;

Randolph, Timothy ;

Rosenzweig, Nicole ;

Srivastava, Shiv ;

Srivastava, Sudhir ;

Thompson, Ian M. ;

Thornquist, Mark ;

Troyer, Dean ;

Yasui, Yutaka ;

Zhang, Zhen ;

Zhu, Liu ;

Semmes, O. John .

CLINICAL CHEMISTRY, 2008, 54 (01) :44-52

[17]

Mitchell BL, 2005, CANCER INFORM, V1, P98

[18] Simple and sensitive quantification of bioactive peptides in biological matrices using liquid chromatography selected reaction monitoring mass spectrometry coupled with trichloroacetic acid clean-up [J].

Murao, Naoaki ;

Ishigai, Masaki ;

Yasuno, Hideyuki ;

Shimonaka, Yasushi ;

Aso, Yoshinori .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2007, 21 (24) :4033-4038

[19] Investigation of human protein variants and their frequency in the general population [J].

Nedelkov, Dobrin ;

Phillips, David A. ;

Tubbs, Kemmons A. ;

Nelson, Randall W. .

MOLECULAR & CELLULAR PROTEOMICS, 2007, 6 (07) :1183-1187

[20] Proteomic analysis of the coagulation reaction in plasma and whole blood using PROTOMAP [J].

Niessen, Sherry ;

Hoover, Heather ;

Gale, Andrew J. .

PROTEOMICS, 2011, 11 (12) :2377-2388

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