The Variability of oxLDL-induced Cytotoxicity on Different Types of Cell Lines

被引:10
作者
Li, Hao [1 ]
Li, Xin Xiang [1 ]
Ma, Qing [2 ]
Cui, Jia [3 ]
机构
[1] Shandong Univ, Qilu Hosp, Dept Tumor Ctr, Jinan 250012, Peoples R China
[2] Shandong Univ, Prov Hosp, Jinan 250012, Peoples R China
[3] Ctr Dis Control & Prevent Shouguang, Shouguang, Peoples R China
关键词
oxLDL; Cytotoxicity; Proliferation; Apoptosis; LOW-DENSITY-LIPOPROTEIN; SERUM TOTAL CHOLESTEROL; ENDOTHELIAL PROGENITOR CELLS; GLYCEMIC INDEX; OXIDIZED LDL; LIPID-PEROXIDATION; CANCER-RISK; MORTALITY; ASSOCIATION; EXPRESSION;
D O I
10.1007/s12013-013-9552-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The epidemiologic studies indicated an association of obesity with increased incidence of colorectal, breast and ovarian cancer. Further studies found a positive correlation between increased serum oxLDL and an increased risk of the three cancers. In contrast, our previous studies found a negative correlation between the serum oxLDL levels and the risk of leukemia and esophageal cancer. Identification of the variability of cytotoxicity of oxLDL-induced on different types of cell lines is important for understanding the mechanism of oxLDL involved in the tumorigenesis. In the present study, we investigated the effective impacts of oxLDL on the proliferation and apoptosis for the human umbilical vein endothelial cells (HUVEC) and two cancer cell lines (EC-9706 and K562/AO2 with multi-drug resistance). HUVEC, K562/AO2 and EC-9706 cell lines were cultured in the presence of oxLDL, and cell proliferation was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, apoptosis and cell cycle by flow cytometer, mRNA expression by RT-PCR and protein expression by Western blot. OxLDL could inhibit proliferation and apoptosis of the three cell lines; however, there were significant differences of effective action on the viability and apoptosis. The dose of oxLDL-induced cytotoxicity on HUVEC was higher than that on the two tumor cells. The antibody of lectin-like oxLDL receptor-1 (LOX-1-ab) can block oxLDL-induced cytotoxicity. Cells apoptosis is mediated by reducing Bcl-2 and increasing Bax and caspase-3 mRNA and protein expression. This study showed the dose of oxLDL-induced cytotoxicity on HUVEC was higher than that on K562/AO2 and EC-9706 tumor cells. The antibody of LOX-1 receptor can block the oxLDL-induced cytotoxicity.
引用
收藏
页码:635 / 644
页数:10
相关论文
共 52 条
  • [1] [Anonymous], 1986, NEW ENGL J MED, V315, P405
  • [2] Circulating soluble lectin-like oxidized low-density lipoprotein receptor-1 levels are associated with proximal/middle segment of the LAD lesions in patients with stable coronary artery disease
    Balin, Mehmet
    Celik, Ahmet
    Kobat, M. Ali
    [J]. CLINICAL RESEARCH IN CARDIOLOGY, 2012, 101 (04) : 247 - 253
  • [3] Chung FL, 2000, CANCER RES, V60, P1507
  • [4] The BCL2 family: Regulators of the cellular life-or-death switch
    Cory, S
    Adams, JM
    [J]. NATURE REVIEWS CANCER, 2002, 2 (09) : 647 - 656
  • [5] ROLE OF SMOKING IN THE U-SHAPED RELATION OF CHOLESTEROL TO MORTALITY IN MEN - THE FRAMINGHAM-STUDY
    DAGOSTINO, RB
    BELANGER, AJ
    KANNEL, WB
    HIGGINS, M
    [J]. AMERICAN JOURNAL OF EPIDEMIOLOGY, 1995, 141 (09) : 822 - 827
  • [6] Small concentrations of oxLDL induce capillary tube formation from endothelial cells via LOX-1-dependent redox-sensitive pathway
    Dandapat, Abhijit
    Hu, Changping
    Sun, Liuqin
    Mehta, Jawahar L.
    [J]. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2007, 27 (11) : 2435 - 2442
  • [7] Oxidized LDL, serum oxidizability and serum lipid levels in patients with breast or ovarian cancer
    Delimaris, Loannis
    Faviou, Elsa
    Antonakos, Georgios
    Stathopoulou, Eleni
    Zachari, Arladni
    Dionyssiou-Asteriou, Amalia
    [J]. CLINICAL BIOCHEMISTRY, 2007, 40 (15) : 1129 - 1134
  • [8] Association of serum levels of lipid and its novel constituents with the different stages of esophageal carcinoma
    Diao, Yutao
    Li, Hao
    Li, Huiqing
    Zhou, Yingzhi
    Ma, Qing
    Wang, Yan
    Li, Dong
    [J]. LIPIDS IN HEALTH AND DISEASE, 2009, 8
  • [9] Anti-LOX-1 therapy in rats with diabetes and dyslipidemia: ablation of renal vascular and epithelial manifestations
    Dominguez, Jesus H.
    Mehta, Jawahar L.
    Li, Dayuan
    Wu, Pengfei
    Kelly, Katherine J.
    Packer, C. Subah
    Temm, Constance
    Goss, Erin
    Cheng, Liang
    Zhang, Shaobo
    Patterson, Carolyn E.
    Hawes, John W.
    Peterson, Richard
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 2008, 294 (01) : F110 - F119
  • [10] Lipid peroxidation cannot be used as a universal criterion of oxidative stress
    Dotan, Y
    Lichtenberg, D
    Pinchuk, I
    [J]. PROGRESS IN LIPID RESEARCH, 2004, 43 (03) : 200 - 227