Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyalkanoates) copolymer from sugars by recombinant Ralstonia eutropha harboring the phaC1Ps and the phaGPs genes of Pseudomonas sp 61-3

被引：35
作者：
Matsumoto, K
Nakae, S
Taguchi, K
Matsusaki, H
Seki, M
Doi, Y
机构：
[1] Riken Inst Phys & Chem Res, Polymer Chem Lab, Wako, Saitama 3510198, Japan
[2] Univ Tokyo, Grad Sch Engn, Dept Chem & Biotechnol, Bunkyo Ku, Tokyo 1138656, Japan
来源：
BIOMACROMOLECULES | 2001年 / 2卷 / 03期
关键词：
D O I：
10.1021/bm0155367
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Heterologous expression of the phaGp(s) and the phaC1p(s) genes encoding 3-hydroxyacyl acyl carrier protein-coenzyme A transacylase and polyhydroxyalkanoate (PHA) synthase from Pseudomonas sp. 61-3, respectively, was performed in the phbC(Re) negative mutant, Ralstonia eutropha PHB(-)4. The recombinant strain of the R. eutropha PHB-4 produced PHA copolymers consisting of 3-hydroxybutyrate (3HB) and medium-chain-length 3-hydroxyalkanoate (mcl-3HA) units of 6-12 carbon atoms from sugars. The 31113 fraction in copolymers was very high (95-97 mol %). The PHA content in the recombinant strain could further be increased by the additional introduction of the phbAB(Re), genes from R. eutropha encoding beta-ketothiolase and NADPH-depedent acetoacetyl-coenzyme A reductase. Differential scanning calorimetry analysis of the PHA copolymers produced by the recombinant R. eutropha PHB(-)4 have indicated that the PHA is a random copolymer of 3HB and mcl-3HA units.
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页码：934 / 939
页数：6
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