Allelic inhibition of displacement activity: A simplified one tube allele-specific PCR for evaluation of ITPA polymorphisms

被引：3

作者：

Galmozzi, E. ^{[1
]}

Facchetti, F. ^{[1
]}

Degasperi, E. ^{[1
]}

Aghemo, A. ^{[1
]}

Lampertico, P. ^{[1
]}

机构：

[1] Univ Milan, Fdn IRCCS Ca Granda Osped Maggiore Policlin, Div Gastroenterol 1, Milan, Italy

来源：

JOURNAL OF VIROLOGICAL METHODS | 2013年 / 187卷 / 02期

关键词：

Hepatitis C virus; Inosine triphosphatase; Allelic discrimination PCR; Ribavirin; VARIANTS; RIBAVIRIN; PROTECT; ANEMIA;

D O I：

10.1016/j.jviromet.2012.11.026

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Recently, genome-wide association studies (GWAS) in patients with chronic hepatitis C virus (HCV) infection have identified two functional single nucleotide polymorphisms (SNPs) in the inosine triphosphatase (ITPA) gene, that are associated strongly and independently with hemolytic anemia in patients exposed to pegylated-interferon (Peg-IFN) plus ribavirin (RBV) combined therapy. Here has been developed a simplified allele discrimination polymerase chain reaction (PCR) assay named allelic inhibition of displacement activity (AIDA) for evaluation of ITPA polymorphisms. AIDA system relies on three unlabeled primers only, two outer common primers and one inner primer with allele-specific 3' terminus mismatch. DNA samples from 192 patients with chronic HCV infection were used to validate the AIDA system and results were compared with the gold standard TaqMan (R) SNP genotyping assay. Concordant data were obtained for all samples, granting for high specificity of the method. In conclusion, AIDA is a practical one-tube method to reproducibly and to assess accurately rs7270101 and rs1127354 ITPA SNPs. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：271 / 273

页数：3

共 12 条

[11] Variants in the ITPA Gene Protect Against Ribavirin-Induced Hemolytic Anemia and Decrease the Need for Ribavirin Dose Reduction
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