Immunolocalization and effect of low concentrations of Insulin like growth factor-1 (IGF-1) in the canine ovary

被引:5
作者
Cardilli, Diogo J. [1 ,8 ]
Sousa-Oliveira, Kellen [2 ]
Franchi-Joao, Carolina [3 ]
Azevedo-Voorwald, Faviana [4 ]
Machado-Silva, Marco A. [5 ]
Oliveira, Joao Ademir [6 ]
Sanchez-Calabuig, Maria Jesus [1 ]
Toniollo, Gilson H. [7 ]
Perez-Gutierrez, Jose F. [1 ]
机构
[1] Univ Complutense Madrid, Fac Vet, Dept Med & Cirugia Anim, Av Puerta Hierro SN, Madrid 28040, Spain
[2] Univ Fed Goias, Escola Vet & Zootecnia, Dept Zootecnia, Goiania, Go, Brazil
[3] Univ Fed Para, Inst Med Vet, Castanhal, PA, Brazil
[4] Univ Estadual Paulista, Fac Ciencias Agr & Vet, Jaboticabal, Brazil
[5] Univ Fed Goias, Escola Vet & Zootecnia, Dept Med Vet, Goiania, Go, Brazil
[6] Univ Estadual Paulista, Fac Ciencias & Agr Vet, Dept Ciencias Exatas, Jaboticabal, Brazil
[7] Univ Estadual Paulista, Fac Ciencias & Agr Vet, Dept Med Vet Prevent & Reprod Anim, Jaboticabal, Brazil
[8] Minist Agr Pecuaria & Abastecimento, Dept Inspecao Prod Origem Anim, Unidade Tecn Reg Uberlandia, Uberlandia, MG, Brazil
关键词
bitch; insulin like growth factor-1; maturation; oocyte; VITRO MATURATION; ESTROUS-CYCLE; OOCYTE MATURATION; LUTEINIZING-HORMONE; NUCLEAR MATURATION; STEROID-PRODUCTION; GRANULOSA; EXPRESSION; PROGESTERONE; RECEPTOR;
D O I
10.1002/vms3.347
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Insulin like growth factor-1 (IGF-1) plays an important role in the regulation of ovarian function. Despite its extensive study in several species, there is a paucity of information about IGF-1`s function and localization in the canine ovary. The aim of the present study was to assess the effect of IGF-1 on oocyte nuclear maturation and to immunolocalize the IGF-1 and its receptor (IGF-1R) in the ovary. Cumulus-oocyte complexes (COCs) were obtained from 34 bitches. The COCs from each bitch were incubated in TCM 199-HEPES in the absence (n = 199) or presence (n = 204) of 100 ng/ml IGF-1 for 96 hr at 38oC in 5% CO2, stained and evaluated for nuclear maturation by fluorescence microscopy. The results showed that the addition of IGF-1 did not have an effect (p > 0.05) on the nuclear maturation under these conditions. The immunohistochemical study revealed nuclear and cytoplasmic staining for IGF-1 and IGF-1R, respectively. Both were localized in all ovarian structures including the corpus luteum, but not in the granulosa cells from primordial follicles. In addition, IGF-1 was not localized in the oocytes in tertiary follicles. The results obtained show the presence of IGF-1 through the stages of follicular growth and in the corpus luteum of the canine ovary. However, its role on oocyte nuclear maturation could not be demonstrated.
引用
收藏
页码:46 / 56
页数:11
相关论文
共 52 条
  • [1] INVIVO REGULATION OF GRANULOSA-CELL SOMATOMEDIN-C INSULIN-LIKE GROWTH FACTOR-I RECEPTORS
    ADASHI, EY
    RESNICK, CE
    HERNANDEZ, ER
    SVOBODA, ME
    VANWYK, JJ
    [J]. ENDOCRINOLOGY, 1988, 122 (04) : 1383 - 1389
  • [2] Histological and immunohistochemical evaluation of canine ovary
    Akihara, Y.
    Shimoyama, Y.
    Kawasako, K.
    Komine, M.
    Hirayama, K.
    Terasawa, A.
    Ohmachi, T.
    Matsuda, K.
    Okamoto, M.
    Taniyama, H.
    [J]. REPRODUCTION IN DOMESTIC ANIMALS, 2007, 42 (05) : 495 - 501
  • [3] Type 1 Insulin-like Growth Factor Receptor Translocates to the Nucleus of Human Tumor Cells
    Aleksic, Tamara
    Chitnis, Meenali M.
    Perestenko, Olga V.
    Gao, Shan
    Thomas, Peter H.
    Turner, Gareth D.
    Protheroe, Andrew S.
    Howarth, Mark
    Macaulay, Valentine M.
    [J]. CANCER RESEARCH, 2010, 70 (16) : 6412 - 6419
  • [4] Immunohistochemical identification of VEGF and IGF-1 in ovaries of bitches on anestrus and estrus
    Almeida, M. V. D.
    Castro, D. S.
    Rial, L. C.
    Lustosa, J. P.
    Bicalho, A. L. F.
    Ortega, H. H.
    Salvetti, N. R.
    Caliari, M. V.
    Vieira, F. G.
    Valle, G. R.
    [J]. ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA, 2015, 67 (02) : 334 - 342
  • [5] Effects of Hormonal Supplementation on Nuclear Maturation and Cortical Granules Distribution of Canine Oocytes During Various Reproductive Stages
    Apparicio, M.
    Alves, A. E.
    Pires-Butler, E. A.
    Ribeiro, A. P. C.
    Covizzi, G. J.
    Vicente, W. R. R.
    [J]. REPRODUCTION IN DOMESTIC ANIMALS, 2011, 46 (05) : 896 - 903
  • [6] Expression of insulin-like growth factor 1 and its receptor in preovulatory follicles and in the corpus luteum in the bitch
    Balogh, Orsolya
    Muller, Linda
    Boos, Alois
    Kowalewski, Mariusz P.
    Reichler, Iris M.
    [J]. GENERAL AND COMPARATIVE ENDOCRINOLOGY, 2018, 269 : 68 - 74
  • [7] Apoptosis of cultured granulosa-lutein cells is reduced by insulin-like growth factor I and may correlate with embryo fragmentation and pregnancy rate
    Bencomo, E
    Pérez, R
    Arteaga, MF
    Acosta, E
    Peña, O
    Lopez, L
    Avila, J
    Palumbo, A
    [J]. FERTILITY AND STERILITY, 2006, 85 (02) : 474 - 480
  • [8] The canine oocyte: uncommon features of in vivo and in vitro maturation
    Chastant-Maillard, Sylvie
    de Lesegno, Christine Viaris
    Chebrout, Martine
    Thoumire, Sandra
    Meylheuc, Thierry
    Fontbonne, Alain
    Chodkiewicz, Marc
    Saint-Dizier, Marie
    Reynaud, Karine
    [J]. REPRODUCTION FERTILITY AND DEVELOPMENT, 2011, 23 (03) : 391 - 402
  • [9] The role of insulin-like growth factor-I (IGF-I) and estradiol in rabbit corpus luteum progesterone production
    Chen, SH
    Zanagnolo, V
    Preutthipan, S
    Roberts, KP
    Goodman, SB
    Dharmarajan, AM
    [J]. ENDOCRINE, 1997, 6 (01) : 73 - 77
  • [10] OVARIAN CYCLE OF BITCH - PLASMA ESTROGEN, LH AND PROGESTERONE
    CONCANNON, PW
    HANSEL, W
    VISEK, WJ
    [J]. BIOLOGY OF REPRODUCTION, 1975, 13 (01) : 112 - 121