Evidence for Novel Hepaciviruses in Rodents

被引：162

作者：

Drexler, Jan Felix ^{[1
]}

Corman, Victor Max ^{[1
]}

Mueller, Marcel Alexander ^{[1
]}

Lukashev, Alexander N. ^{[2
]}

Gmyl, Anatoly ^{[2
,3
]}

Coutard, Bruno ^{[4
,5
]}

Adam, Alexander ^{[6
]}

Ritz, Daniel ^{[1
]}

Leijten, Lonneke M. ^{[7
]}

van Riel, Debby ^{[7
]}

Kallies, Rene ^{[1
]}

Klose, Stefan M.

Gloza-Rausch, Florian ^{[1
,9
]}

Binger, Tabea ^{[1
]}

Annan, Augustina ^{[10
]}

Adu-Sarkodie, Yaw ^{[11
]}

Oppong, Samuel ^{[11
]}

Bourgarel, Mathieu ^{[12
]}

Rupp, Daniel ^{[13
]}

Hoffmann, Bernd ^{[14
]}

Schlegel, Mathias ^{[15
]}

Kuemmerer, Beate M. ^{[1
]}

Krueger, Detlev H. ^{[16
]}

Schmidt-Chanasit, Jonas ^{[17
]}

Aguilar Setien, Alvaro ^{[18
]}

Cottontail, Veronika M. ^{[8
]}

Hemachudha, Thiravat ^{[19
]}

Wacharapluesadee, Supaporn ^{[19
]}

Osterrieder, Klaus ^{[20
]}

Bartenschlager, Ralf ^{[13
]}

Matthee, Sonja ^{[21
]}

Beer, Martin ^{[14
]}

Kuiken, Thijs ^{[7
]}

Reusken, Chantal ^{[22
]}

Leroy, Eric M. ^{[23
,24
]}

Ulrich, Rainer G. ^{[15
]}

Drosten, Christian ^{[1
]}

机构：

[1] Univ Bonn, Med Ctr, Inst Virol, Bonn, Germany

[2] Chumakov Inst Poliomyelitis & Viral Encephalitide, Moscow, Russia

[3] Moscow MV Lomonosov State Univ, Moscow, Russia

[4] CNRS, UMR 7257, Marseille, France

[5] Aix Marseille Univ, Marseille, France

[6] Univ Cologne, Med Ctr, Inst Pathol, D-50931 Cologne, Germany

[7] Erasmus MC, Dept Virosci, Rotterdam, Netherlands

[8] Univ Ulm, Inst Expt Ecol, D-89069 Ulm, Germany

[9] Noctalis, Ctr Bat Protect & Informat, Bad Segeberg, Germany

[10] Kumasi Ctr Collaborat Res Trop Med KCCR, Kumasi, Ghana

[11] Kwame Nkrumah Univ Sci & Technol, Kumasi, Ghana

[12] UPR AGIRs, Ctr Cooperat Int Rech Agron Dev, Montpellier, France

[13] Heidelberg Univ, Med Facil, Dept Infect Dis, Heidelberg, Germany

[14] Friedrich Loeffler Inst, Inst Virus Diagnost, Greifswald, Germany

[15] Friedrich Loeffler Inst, Inst Novel & Emerging Infect Dis, Greifswald, Germany

[16] Charite, Inst Med Virol, Berlin, Germany

[17] Bernhard Nocht Inst Trop Med, Dept Virol, D-20359 Hamburg, Germany

[18] Hosp Pediatri, Unidad Invest Med Inmunol, Mexico City, DF, Mexico

[19] Chulalongkorn Univ, Fac Med, Neurosci Ctr Res & Dev, Bangkok 10330, Thailand

[20] Free Univ Berlin, Inst Virol, Dept Vet Med, Berlin, Germany

[21] Univ Stellenbosch, Dept Conservat Ecol & Entomol, ZA-7600 Stellenbosch, South Africa

[22] Netherlands Ctr Infect Dis Control, Bilthoven, Netherlands

[23] Ctr Int Rech Med Franceville, Franceville, Gabon

[24] IRD CNRS UM1, UMR MIVEGEC 224, Inst Rech Dev, Montpellier, France

来源：

PLOS PATHOGENS | 2013年 / 9卷 / 06期

关键词：

HEPATITIS-C VIRUS; NEW-WORLD MONKEYS; EXPERIMENTAL-INFECTION; SEQUENCE-ANALYSIS; HCV INFECTION; MODEL; IDENTIFICATION; EMERGENCE; GENUS; BATS;

D O I：

10.1371/journal.ppat.1003438

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Hepatitis C virus (HCV) is among the most relevant causes of liver cirrhosis and hepatocellular carcinoma. Research is complicated by a lack of accessible small animal models. The systematic investigation of viruses of small mammals could guide efforts to establish such models, while providing insight into viral evolutionary biology. We have assembled the so-far largest collection of small-mammal samples from around the world, qualified to be screened for bloodborne viruses, including sera and organs from 4,770 rodents (41 species); and sera from 2,939 bats (51 species). Three highly divergent rodent hepacivirus clades were detected in 27 (1.8%) of 1,465 European bank voles (Myodes glareolus) and 10 (1.9%) of 518 South African four-striped mice (Rhabdomys pumilio). Bats showed anti-HCV immunoblot reactivities but no virus detection, although the genetic relatedness suggested by the serologic results should have enabled RNA detection using the broadly reactive PCR assays developed for this study. 210 horses and 858 cats and dogs were tested, yielding further horse-associated hepaciviruses but none in dogs or cats. The rodent viruses were equidistant to HCV, exceeding by far the diversity of HCV and the canine/equine hepaciviruses taken together. Five full genomes were sequenced, representing all viral lineages. Salient genome features and distance criteria supported classification of all viruses as hepaciviruses. Quantitative RT-PCR, RNA in-situ hybridisation, and histopathology suggested hepatic tropism with liver inflammation resembling hepatitis C. Recombinant serology for two distinct hepacivirus lineages in 97 bank voles identified seroprevalence rates of 8.3 and 12.4%, respectively. Antibodies in bank vole sera neither cross-reacted with HCV, nor the heterologous bank vole hepacivirus. Co-occurrence of RNA and antibodies was found in 3 of 57 PCR-positive bank vole sera (5.3%). Our data enable new hypotheses regarding HCV evolution and encourage efforts to develop rodent surrogate models for HCV.

引用

页数：17

共 82 条

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