Metagenomic Approaches for Exploiting Uncultivated Bacteria as a Resource for Novel Biosynthetic Enzymology

被引：95

作者：

Wilson, Micheal C. ^{[1
]}

Piel, Joern ^{[1
]}

机构：

[1] ETH, Inst Microbiol, CH-8093 Zurich, Switzerland

来源：

CHEMISTRY & BIOLOGY | 2013年 / 20卷 / 05期

关键词：

SINGLE-CELL GENOMICS; POLYKETIDE SYNTHASE GENE; BRYOZOAN BUGULA-NERITINA; ENVIRONMENTAL DNA; NATURAL-PRODUCTS; HETEROLOGOUS EXPRESSION; PEPTIDE SYNTHETASE; UNCULTURED MICROORGANISMS; ESCHERICHIA-COLI; SECONDARY METABOLITES;

D O I：

10.1016/j.chembiol.2013.04.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Most biologically active microbial natural products are known from strains that can be isolated and cultivated in the laboratory. However, the genomics era has revealed that cultured bacteria represent a mere fraction of total estimated bacterial biodiversity. With the development of community genomics, termed metagenomics, the uncultivated majority became accessible for functional analysis. Through metagenomic studies, novel biocatalysts and biosynthetic pathways are being discovered at a pace previously not possible using traditional molecular biology techniques. Additionally, the study of uncultivated bacteria has provided valuable insights into previously overlooked biocatalysts from cultured strains. This perspective highlights recent discoveries from metagenomics of uncultivated bacteria and discusses the impact of those findings on the field of natural products.

引用

页码：636 / 647

页数：12

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