Synaptic clustering of AMPA receptors by the extracellular immediate-early gene product Narp

被引：364

作者：

O'Brien, RJ

Xu, DS

Petralia, RS

Steward, O

Huganir, RL

Worley, P ^{[1
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Neurosci, Baltimore, MD 21205 USA

[2] Johns Hopkins Univ, Sch Med, Howard Hughes Med Inst, Baltimore, MD 21205 USA

[3] Johns Hopkins Univ, Sch Med, Dept Neurol, Baltimore, MD 21205 USA

[4] Natl Inst Deafness & Commun Disorders, NIH, Neurochem Lab, Bethesda, MD 20892 USA

[5] Univ Virginia, Dept Neurosci, Charlottesville, VA 22908 USA

来源：

NEURON | 1999年 / 23卷 / 02期

关键词：

D O I：

10.1016/S0896-6273(00)80782-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Narp (neuronal activity-regulated pentraxin) is a secreted immediate-early gene (IEG) regulated by synaptic activity in brain. In this study, we demonstrate that Narp possesses several properties that make it likely to play a key role in excitatory synaptogenesis. Narp is shown to be selectively enriched at excitatory synapses on neurons from both the hippocampus and spinal cord. Overexpression of recombinant Narp increases the number of excitatory but not inhibitory synapses in cultured spinal neurons. In transfected HEK 293T cells, Narp interacts with itself, forming large surface clusters that coaggregate AMPA receptor subunits. Moreover, Narp-expressing HEK 293T cells can induce the aggregation of neuronal AMPA receptors. These studies support a model in which Narp functions as an extracellular aggregating factor for AMPA receptors.

引用

页码：309 / 323

页数：15

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