In situ extraction of RNA from marine-derived fungi associated with the marine sponge, Haliclona simulans

被引：0
|
作者：
Baker, Paul W. [1 ]
Dobson, Alan D. W. [2 ]
Marchesi, Julian [3 ]
机构：
[1] Bangor Univ, Sch Environm Nat Resources & Geog, Bangor LL57 2UW, Gwynedd, Wales
[2] Natl Univ Ireland Univ Coll Cork, Environm Res Inst, Cork, Ireland
[3] Cardiff Univ, Sch Biosci, Cardiff CF10 3AT, S Glam, Wales
来源：
MYCOLOGICAL PROGRESS | 2012年 / 11卷 / 04期
关键词：
Haliclona simulans; Marine fungi; RNA; Symbiosis; ENVIRONMENTAL-SAMPLES; RIBOSOMAL-RNA; DIVERSITY; DNA; LIBRARIES;
D O I：
10.1007/s11557-011-0796-4
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Several different methods were compared to recover a significant quantity of high quality RNA from H. simulans containing RNA of marine-derived fungi. Further processing of the RNA showed that amplification products could be obtained using fungal specific primers, RNA enriched in mRNA could be recovered and an oligonucleotide-linker could be attached to cDNA. The potential applications of these methods are discussed for future studies in determining whether marine fungi could be symbiotically associated with marine sponges.
引用
收藏
页码：953 / 956
页数：4
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