NMR resonance assignment of the autoimmunity protein SpaI from Bacillus subtilis ATCC 6633

被引：4

作者：

Christ, Nina Alexandra ^{[1
,2
]}

Duchardt-Ferner, Elke ^{[1
,2
]}

Duesterhus, Stefanie ^{[1
]}

Koetter, Peter ^{[1
]}

Entian, Karl-Dieter ^{[1
]}

Woehnert, Jens ^{[1
,2
]}

机构：

[1] Goethe Univ Frankfurt, Inst Mol Biowissensch, D-60438 Frankfurt, Germany

[2] Goethe Univ Frankfurt, Ctr Biomol Magnet Resonance BMRZ, D-60438 Frankfurt, Germany

来源：

BIOMOLECULAR NMR ASSIGNMENTS | 2012年 / 6卷 / 01期

关键词：

NMR-assignments; Triple resonance experiments; SpaI; Self-immunity; Lantibiotic; Subtilin; CHEMICAL-SHIFTS; LANTIBIOTICS; SPECTROSCOPY; BIOSYNTHESIS; EPIDERMIN; IMMUNITY; PORES; NISIN;

D O I：

10.1007/s12104-011-9314-5

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Bacillus subtilis ATCC 6633 produces the lipid II targeting lantibiotic subtilin. For self-protection these gram-positive bacteria express a cluster of four self-immunity proteins named SpaIFEG. SpaI is a 16.8 kDa lipoprotein which is attached to the outside of the cytoplasmic membrane via a covalently linked diacylglycerol anchor. Together with the ABC-transporter SpaFEG, SpaI protects the membrane from subtilin insertion and there is evidence for a direct interaction of SpaI with subtilin. As a prerequisite for further structural studies of SpaI and the SpaI/subtilin complex we report here the full H-1, N-15, C-13 chemical shift assignment for a stable 14.9 kDa C-terminal fragment of SpaI.

引用

页码：9 / 13

页数：5

共 22 条

[1] Lantibiotics: Mode of Action, Biosynthesis and Bioengineering [J].

Bierbaum, G. ;

Sahl, H. -G. .

CURRENT PHARMACEUTICAL BIOTECHNOLOGY, 2009, 10 (01) :2-18

[2] Use of the cell wall precursor lipid II by a pore-forming peptide antibiotic [J].

Breukink, E ;

Wiedemann, I ;

van Kraaij, C ;

Kuipers, OP ;

Sahl, HG ;

de Kruijff, B .

SCIENCE, 1999, 286 (5448) :2361-2364

[3] Role of lipid-bound peptidoglycan precursors in the formation of pores by nisin, epidermin and other lantibiotics [J].

Brötz, H ;

Josten, M ;

Wiedemann, I ;

Schneider, U ;

Götz, F ;

Bierbaum, G ;

Sahl, HG .

MOLECULAR MICROBIOLOGY, 1998, 30 (02) :317-327

[4] Protein energetic conformational analysis from NMR chemical shifts (PECAN) and its use in determining secondary structural elements [J].

Eghbalnia, HR ;

Wang, LY ;

Bahrami, A ;

Assadi, A ;

Markley, JL .

JOURNAL OF BIOMOLECULAR NMR, 2005, 32 (01) :71-81

[5] REGULATION OF NISIN BIOSYNTHESIS AND IMMUNITY IN LACTOCOCCUS-LACTIS 6F3 [J].

ENGELKE, G ;

GUTOWSKIECKEL, Z ;

KIESAU, P ;

SIEGERS, K ;

HAMMELMANN, M ;

ENTIAN, KD .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (03) :814-825

[6]

Gross E, 1975, PEPTIDES CHEM STRUCT, P31

[7] Maturation and processing of Spal, the lipoprotein involved in subtilin immunity in Bacillus subtilis ATCC 6633 [J].

Halami, Prakash M. ;

Stein, Torsten ;

Chandrashekar, Arun ;

Entian, Karl-Dieter .

MICROBIOLOGICAL RESEARCH, 2010, 165 (03) :183-189

[8]

Keller RLJ, 2004, The computer aided resonance assignment tutorial

[9] GENES INVOLVED IN SELF-PROTECTION AGAINST THE LANTIBIOTIC SUBTILIN PRODUCED BY BACILLUS-SUBTILIS ATCC-6633 [J].

KLEIN, C ;

ENTIAN, KD .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (08) :2793-2801

[10] Recommendations for the presentation of NMR structures of proteins and nucleic acids -: IUPAC-IUBMB-IUPAB Inter-Union Task Group on the Standardization of Data Bases of Protein and Nucleic Acid Structures Determined by NMR Spectroscopy [J].

Markley, JL ;

Bax, A ;

Arata, Y ;

Hilbers, CW ;

Kaptein, R ;

Sykes, BD ;

Wright, PE ;

Wüthrich, K .

JOURNAL OF BIOMOLECULAR NMR, 1998, 12 (01) :1-23

← 1 2 3 →