An alpha class glutathione S-transferase from pike liver

被引：1

作者：

Borvinskaya, E. V. ^{[1
]}

Smirnov, L. P. ^{[1
]}

Nemova, N. N. ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Biol, Karelian Res Ctr, Petrozavodsk 185910, Russia

来源：

RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2013年 / 39卷 / 05期

基金：

俄罗斯基础研究基金会;

关键词：

detoxification enzyme system; glutathione S-transferase; oxidative stress; PLAICE PLEURONECTES-PLATESSA; MOLECULAR-CLONING; 4-NITROQUINOLINE; 1-OXIDE; AFFINITY-CHROMATOGRAPHY; SALMO-SALAR; PURIFICATION; ISOENZYMES; RAT; IDENTIFICATION; INDUCTION;

D O I：

10.1134/S1068162013050051

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glutathione S-transferase (GST) was isolated from the Esox lucius liver and purified to the homogenous state by SDS-PAGE and isoelectrofocusing. It was found to be a homodimer with a subunit molecular weight of 25235.36 Da (HPLC-MS/MS data) and pI of about 6.4. Its substrate specificity, thermal stability, some kinetic characteristics, and optimum pH were studied. The enzyme was identified as Alpha class GST.

引用

页码：498 / 503

页数：6

共 48 条

[1] DIFFERENTIAL ACTIVITY OF HUMAN, RAT, MOUSE AND BACTERIA GLUTATHIONE TRANSFERASE ISOENZYMES TOWARDS 4-NITROQUINOLINE 1-OXIDE [J].

ACETO, A ;

DIILIO, C ;

LOBELLO, M ;

BUCCIARELLI, T ;

ANGELUCCI, S ;

FEDERICI, G .

CARCINOGENESIS, 1990, 11 (12) :2267-2269

[2] PURIFICATION OF MAJOR BASIC GLUTATHIONE TRANSFERASE ISOENZYMES FROM RAT-LIVER BY USE OF AFFINITY-CHROMATOGRAPHY AND FAST PROTEIN LIQUID CHROMATOFOCUSING [J].

ALIN, P ;

JENSSON, H ;

GUTHENBERG, C ;

DANIELSON, UH ;

TAHIR, MK ;

MANNERVIK, B .

ANALYTICAL BIOCHEMISTRY, 1985, 146 (02) :313-320

[3] Purification and characterization of glutathione transferases from the sea bass (Dicentrarchus labrax) liver [J].

Angelucci, S ;

Sacchetta, P ;

Moio, P ;

Melino, S ;

Petruzzelli, R ;

Gervasi, P ;

Di Ilio, C .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2000, 373 (02) :435-441

[4] Marine glutathione S-transferases [J].

Blanchette, Brian ;

Feng, Xia ;

Singh, Bal Ram .

MARINE BIOTECHNOLOGY, 2007, 9 (05) :513-542

[5]

Borvinskaya E.V., 2013, DOKL BIOL SCI, V448, P22

[6] GLUTATHIONE TRANSFERASES IN THE TAPEWORM MONIEZIA-EXPANSA [J].

BROPHY, PM ;

SOUTHAN, C ;

BARRETT, J .

BIOCHEMICAL JOURNAL, 1989, 262 (03) :939-946

[7] Glutathione transferases from Anguilla anguilla liver: Identification, cloning and functional characterization [J].

Carletti, Erminia ;

Sulpizio, Marilisa ;

Bucciarelli, Tonino ;

Del Boccio, Piero ;

Federici, Luca ;

Di Ilio, Carmine .

AQUATIC TOXICOLOGY, 2008, 90 (01) :48-57

[8]

Chasseaud L.F., 1979, ADV CANCER RES, V29, P176

[9] Transfection of mGSTA4 in HL-60 cells protects against 4-hydroxynonenal-induced apoptosis by inhibiting JNK-mediated signaling [J].

Cheng, JZ ;

Singhal, SS ;

Sharma, A ;

Saini, M ;

Yang, YS ;

Awasthi, S ;

Zimniak, P ;

Awasthi, YC .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2001, 392 (02) :197-207

[10] THE HEPATIC GLUTATHIONE TRANSFERASES OF THE MALE LITTLE SKATE, RAJA-ERINACEA [J].

FOUREMAN, GL ;

BEND, JR .

CHEMICO-BIOLOGICAL INTERACTIONS, 1984, 49 (1-2) :89-103

← 1 2 3 4 5 →