Three putative sucrose transporters are differentially expressed in grapevine tissues

被引:128
作者
Davies, C
Wolf, T
Robinson, SP
机构
[1] Cooperat Res Ctr Viticulture, Glen Osmond, SA 5064, Australia
[2] CSIRO, Hort Res Unit, Glen Osmond, SA 5064, Australia
[3] Geisenheim Res Stn, Sect Grapevine Breeding & Grapevine Grafting, D-65366 Geisenheim, Germany
关键词
grape berry; sucrose transporters;
D O I
10.1016/S0168-9452(99)00059-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A total of three full-length cDNA clones (VvSUC11, VvSUC12, VvSUC27) encoding proteins with sequence similarity to membrane located solute transporters have been isolated from grape (Vitis vinifera L.) berries. Sequence analysis indicated that these deduced proteins are most closely related to plant, plasma membrane located, sucrose-H+ symporters. Northern blot analysis showed that these clones are differentially expressed in different tissues. Expression of VvSUC11 and VvSUC12 was upregulated in berries when hexose accumulation commenced in the vacuole while the expression of VvSUC27 declined during ripening. These results indicate a possible role for sucrose transporters in the ripening-associated accumulation of hexoses in the berry vacuole. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:93 / 100
页数:8
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