Midtrimester Fetal Herpes Simplex-2 Diagnosis by Serology, Culture and Quantitative Polymerase Chain Reaction

被引：8

作者：

Curtin, William M. ^{[1
,2
]}

Menegus, Marilyn A. ^{[3
]}

Patru, Maria-Magdalena ^{[3
]}

Peterson, C. Jeanne ^{[2
]}

Metlay, Leon A. ^{[3
]}

Mooney, Robert A. ^{[3
]}

Stanwood, Nancy L. ^{[4
,5
]}

Scheible, Amy L. ^{[2
]}

Dorgan, Angela ^{[2
]}

机构：

[1] Penn State Univ, Milton S Hershey Med Ctr, Penn State Coll Med, Dept Obstet Gynecol & Pathol,Div Maternal Fetal M, Hershey, PA 17033 USA

[2] Univ Rochester, Sch Med & Dent, Div Maternal Fetal Med, Rochester, NY USA

[3] Univ Rochester, Sch Med & Dent, Dept Pathol & Lab Med, Rochester, NY USA

[4] Univ Rochester, Sch Med & Dent, Dept Obstet & Gynecol, Rochester, NY 14642 USA

[5] Yale Univ, Sch Med, Dept Obstet Gynecol & Reprod Sci, New Haven, CT USA

来源：

FETAL DIAGNOSIS AND THERAPY | 2013年 / 33卷 / 02期

关键词：

Prenatal diagnosis; Fetal herpes simplex virus-2; Viral culture; Serology; Quantitative polymerase chain reaction; Elevated maternal serum alpha-fetoprotein; Fetal growth restriction; HERPES-SIMPLEX-VIRUS; PRENATAL-DIAGNOSIS; GENITAL HERPES; INFECTION;

D O I：

10.1159/000342756

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The acquisition of herpes simplex virus (HSV) in utero comprises a minority of neonatal herpes infections. Prenatal diagnosis is rare. We describe a midtrimester diagnosis of fetal HSV-2 infection. Ultrasound at 20 weeks for elevated maternal serum alpha-fetoprotein (MSAFP) showed lagging fetal growth, echogenic bowel, echogenic myocardium, and liver with a mottled pattern of echogenicity. Amniocentesis demonstrated normal karyotype, elevated AFP and positive acetylcholinesterase. Culture isolated HSV-2 with an aberrant growth pattern. Maternal serology was positive for HSV-2. Quantitative DNA polymerase chain reaction (PCR) showed 59 million copies/ml. Fetal autopsy demonstrated widespread tissue necrosis but only sparse HSV-2 inclusions. Fetal HSV-2 infection can be suspected when an elevated MSAFP accompanies ultrasound findings suggesting perinatal infection. Maternal HSV serology, amniotic fluid culture and quantitative PCR are recommended for diagnostic certainty and counseling. Copyright (c) 2012 S. Karger AG, Basel

引用

页码：133 / 136

页数：4

共 11 条

[1] Herpes simplex virus DNA in amniotic fluid without neonatal infection [J].