Immobilization Effects on the Catalytic Properties of Two Fusarium Verticillioides Lipases: Stability, Hydrolysis, Transesterification and Enantioselectivity Improvement

被引:17
作者
Antonio Facchini, Fernanda Dell [1 ]
Pereira, Marita Gimenez [2 ]
Vici, Ana Claudia [2 ]
Filice, Marco [3 ]
Costa Pessela, Benevides [4 ]
Manuel Guisan, Jose [5 ]
Fernandez-Lorente, Gloria [4 ]
Teixeira de Moraes Polizeli, Maria de Lourdes [2 ]
机构
[1] Univ Sao Paulo, Dept Med, Fac Med Ribeirao Preto, BR-14040900 Sao Paulo, Brazil
[2] Univ Sao Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Sao Paulo, Brazil
[3] Spanish Natl Ctr Cardiovasc Res, CNIC, Nanobiotechnol Mol Imaging & Metabol Grp, Madrid 28029, Spain
[4] CSIC, CIAL, Inst Ciencias Alimentac, Dept Biotecnol & Microbiol Alimentos, Calle Nicolas Cabrera 9,CampusUAM, Canto Blanco 28049, Spain
[5] CSIC, Inst Catalisis & Petroleoquim, Dept Biocatalisis, Campus UAM, Canto Blanco 28049, Spain
来源
CATALYSTS | 2018年 / 8卷 / 02期
基金
巴西圣保罗研究基金会;
关键词
immobilization; purification; lipase; Fusarium verticillioides; EPA; DHA; MULTIPOINT COVALENT IMMOBILIZATION; RHIZOMUCOR-MIEHEI LIPASE; ENZYME-KINETIC DATA; FISH-OIL; HYDROPHOBIC SUPPORTS; BIODIESEL PRODUCTION; BIOCHEMICAL-CHARACTERIZATION; HYPOCREA-PSEUDOKONINGII; INTERFACIAL ADSORPTION; EXTRACELLULAR LIPASE;
D O I
10.3390/catal8020084
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Fusarium verticillioides lipases were purified in a "cascade" method using octadecyl Sepabeads and octyl Sepharose resins, which led to the isolation of two proteins with lipolytic activities. Lip 1 was purified after octyl Sepharose adsorption presenting 30.3 kDa and, Lip 2 presented 68.0 kDa after octadecyl adsorption. These immobilization processes resulted in an increase of 3-fold in activity of each immobilized enzyme. These enzymes presented optima of pH of 5.0 and 6.0, respectively and temperature at 40 degrees C. They were thermostable at 40 degrees C and both remained more than 50% of its activity at the pH range of 5.0 to 7.0, with 180 min of incubation. The sardine oil hydrolysis showed higher EPA/DHA ratio. Concerning the ethanolysis reaction, Lip 2 showed higher conversion (5.5%) and both lipases showed activity in the release of the S enantiomers from 2-O-butyryl-2-phenylacetic acid (mandelic butyrate acid) and HPBE hydrolysis. Lip 2 also demonstrated capacity of transesterification. These applications made these enzymes attractive for industrial application.
引用
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页数:17
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