Phlorotannin Extracts from Fucales Characterized by HPLC-DAD-ESI-MSn: Approaches to Hyaluronidase Inhibitory Capacity and Antioxidant Properties

被引:173
作者
Ferreres, Federico [1 ]
Lopes, Graciliana [2 ]
Gil-Izquierdo, Angel [1 ]
Andrade, Paula B. [2 ]
Sousa, Carla [2 ]
Mouga, Teresa [3 ]
Valentao, Patricia [2 ]
机构
[1] CEBAS CSIC, Dept Food Sci & Technol, Res Grp Qual Safety & Bioact Plant Foods, Murcia, Spain
[2] Univ Porto, Fac Pharm, Dept Chem, REQUIMTE Lab Pharmacognosy, P-4050313 Oporto, Portugal
[3] Polytech Inst Leiria, Sch Tourism & Maritime Technol, GIRM Marine Resources Res Grp, P-2524909 Peniche, Portugal
来源
MARINE DRUGS | 2012年 / 10卷 / 12期
关键词
phlorotannins; skin aging; hyaluronidase; antioxidant activity; HPLC-DAD-ESI-MSn; BROWN ALGAL PHLOROTANNINS; OXIDATIVE STRESS; FUCUS-VESICULOSUS; MACROALGAE; ASSAY;
D O I
10.3390/md10122766
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Purified phlorotannin extracts from four brown seaweeds (Cystoseira nodicaulis (Withering) M. Roberts, Cystoseira tamariscifolia (Hudson) Papenfuss, Cystoseira usneoides (Linnaeus) M. Roberts and Fucus spiralis Linnaeus), were characterized by HPLC-DAD-ESI-MSn. Fucophloroethol, fucodiphloroethol, fucotriphloroethol, 7-phloroeckol, phlorofucofuroeckol and bieckol/dieckol were identified. The antioxidant activity and the hyaluronidase (HAase) inhibitory capacity exhibited by the extracts were also assessed. A correlation between the extracts activity and their chemical composition was established. F. spiralis, the species presenting higher molecular weight phlorotannins, generally displayed the strongest lipid peroxidation inhibitory activity (IC50 = 2.32 mg/mL dry weight) and the strongest HAase inhibitory capacity (IC50 = 0.73 mg/mL dry weight). As for superoxide radical scavenging, C. nodicaulis was the most efficient species (IC50 = 0.93 mg/mL dry weight), followed by F. spiralis (IC50 = 1.30 mg/mL dry weight). These results show that purified phlorotannin extracts have potent capabilities for preventing and slowing down the skin aging process, which is mainly associated with free radical damage and with the reduction of hyaluronic acid concentration, characteristic of the process.
引用
收藏
页码:2766 / 2781
页数:16
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