Oxidative DNA cleavage, cytotoxicity and antimicrobial studies of L-ornithine copper (II) complexes

被引：19

作者：

Chetana, P. R. ^{[1
]}

Rao, Ramakrishna ^{[1
]}

Saha, Sounik ^{[2
]}

Policegoudra, R. S. ^{[3
]}

Vijayan, P. ^{[4
]}

Aradhya, M. S. ^{[5
]}

机构：

[1] Bangalore Univ, Dept Chem, Bangalore 560001, Karnataka, India

[2] Indian Inst Sci, Dept Inorgan & Phys Chem, Bangalore 560012, Karnataka, India

[3] Def Res Lab, Dept Biotechnol, Tezpur 784001, India

[4] JSS Coll Pharm, Ooty 643001, India

[5] CFTRI, Dept Fruit & Vegetable Technol, Mysore 570020, Karnataka, India

来源：

POLYHEDRON | 2012年 / 48卷 / 01期

关键词：

Ornithine; Copper (II); DNA cleavage; A-549; HEp-2; Antibacterial activity; INDUCED NUCLEASE ACTIVITY; ALPHA-AMINO-ACIDS; LIGHT-INDUCED DNA; OXOVANADIUM(IV) COMPLEXES; DECARBOXYLASE ACTIVITY; TITANOCENE DICHLORIDE; ANTIFUNGAL ACTIVITY; ETHIDIUM-BROMIDE; L-METHIONINE; BINDING;

D O I：

10.1016/j.poly.2012.08.081

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

New ternary copper (II) complexes, [Cu(L-orn)(B)(Cl)](Cl center dot 2H(2)O) (1-2) where L-orn is L-ornithine, B is an N,N-donor heterocyclic base, viz. 2,2'-bipyridine (bpy, 1) and 1,10-phenanthroline (phen, 2), were synthesized and characterized by various spectroscopic techniques. Complex 2 is characterized by the X-ray single crystallographic method. The complex shows a distorted square-pyramidal (4 + 1) CuN3OCl coordination sphere. Binding interactions of the complexes with calf thymus DNA (CT-DNA) were investigated by UV-Vis absorption titration, ethidium bromide displacement assay, viscometric titration experiment and DNA melting studies. Complex 2 shows appreciable chemical nuclease activity in the presence of 3-mercaptopropionic acid (MPA). The complexes were subjected to in vitro cytotoxicity studies against carcinomic human alveolar basal epithelial cells (A-549) and human epithelial (HEp-2) cells. The IC50 values of 1 and 2 are less than that of cisplatin against HEp-2 cell lines. MIC values for 1 against the bacterial strains Streptococcus mutans and Pseudomonas aeruginosa are 0.5 mM. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：43 / 50

页数：8

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