Fluorescence Excitation Spectra from Individual Chlorosomes of the Green Sulfur Bacterium Chlorobaculum tepidum

被引：20

作者：

Jendrny, Marc ^{[1
]}

Aartsma, Thijs J. ^{[2
]}

Koehler, Juergen ^{[1
]}

机构：

[1] Univ Bayreuth, Bayreuth Inst Macromol Res BIMF, D-95447 Bayreuth, Germany

[2] Leiden Univ, Leiden Inst Phys, NL-2333 CA Leiden, Netherlands

来源：

JOURNAL OF PHYSICAL CHEMISTRY LETTERS | 2012年 / 3卷 / 24期

关键词：

LIGHT-HARVESTING CHLOROSOMES; CHLOROBIUM-TEPIDUM; PHOTOSYNTHETIC BACTERIA; BACTERIOCHLOROPHYLL-C; ENERGY-TRANSFER; ORGANIZATION; MICROSCOPY; SPECTROSCOPY; NANOTUBES; LIMICOLA;

D O I：

10.1021/jz301808h

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We performed polarization-resolved fluorescence excitation spectroscopy on individual chlorosomes from the photosynthetic green sulfur bacterium Chlorobaculum tepidum. The experiments were conducted at room temperature and under cryogenic conditions. All spectra showed a strong intensity modulation as a function of the polarization of the incident radiation, and we determined the modulation ratio as a function of the excitation energy. Under ambient conditions this ratio shows only little variation across the absorption band, whereas the low-temperature experiments clearly revealed. that the broad absorption band around 740 nm consists of several spectral contributions.

引用

页码：3745 / 3750

页数：6

共 39 条

[1] An obligately photosynthetic bacterial anaerobe from a deep-sea hydrothermal vent [J].

Beatty, JT ;

Overmann, J ;

Lince, MT ;

Manske, AK ;

Lang, AS ;

Blankenship, RE ;

Van Dover, CL ;

Martinson, TA ;

Plumley, FG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (26) :9306-9310

[2]

Blankenship R.E., 1995, ANOXYGENIC PHOTOSYNT

[3]

de Ruijter W. P. F., 2005, THESIS U LEIDEN

[4] Excitons in tubular molecular aggregates [J].

Didraga, C ;

Knoester, J .

JOURNAL OF LUMINESCENCE, 2004, 110 (04) :239-245

[5] Optical spectra and localization of excitons in inhomogeneous helical cylindrical aggregates [J].

Didraga, C ;

Knoester, J .

JOURNAL OF CHEMICAL PHYSICS, 2004, 121 (21) :10687-10698

[6] Chiral exciton wave functions in cylindrical J aggregates [J].

Didraga, C ;

Knoester, J .

JOURNAL OF CHEMICAL PHYSICS, 2004, 121 (02) :946-959

[7] Optical properties of helical cylindrical molecular aggregates: The homogeneous limit [J].

Didraga, C ;

Klugkist, JA ;

Knoester, J .

JOURNAL OF PHYSICAL CHEMISTRY B, 2002, 106 (44) :11474-11486

[8]

Dorssen J., 1986, PHOTOSYNTH RES, V9, P33

[9]

Eisele DM, 2012, NAT CHEM, V4, P655, DOI [10.1038/nchem.1380, 10.1038/NCHEM.1380]

[10] Chlorobium tepidum:: insights into the structure, physiology, and metabolism of a green sulfur bacterium derived from the complete genome sequence [J].

Frigaard, NU ;

Chew, AGM ;

Li, H ;

Maresca, JA ;

Bryant, DA .

PHOTOSYNTHESIS RESEARCH, 2003, 78 (02) :93-117

← 1 2 3 4 →