Reactive oxygen species activate HIV long terminal repeat via post-translational control of NF-κB

被引:43
|
作者
Pyo, Chul-Woong [1 ]
Yang, Young Lae [1 ]
Yoo, Na-Kyung [1 ]
Choi, Sang-Yun [1 ]
机构
[1] Korea Univ, Sch Life Sci & Biotechnol, Seoul 136701, South Korea
关键词
oxidative stress; HIV; LTR; NF-kappa B; H2O2;
D O I
10.1016/j.bbrc.2008.08.114
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Reduction/oxidation disorder is one of the most common ailments in HIV-infected patients, and such patients are frequently left exposed to chronic oxidative stress after the generation of reactive oxygen species (ROS). Although a variety of clinical trials to inhibit HIV infection have been conducted by focusing on oxidative stress, their precise targets and reaction mechanism have remained unclear. In this study, we demonstrate that H2O2 treatment strongly induced HIV long terminal repeat (LTR)-driven luciferase expression in Jurkat T lymphocytes via NF-kappa B activation. Treatment with the SN50 peptide or the mutation of NF-kappa B binding site on LTR resulted in impaired LTR activity in response to ROS. H2O2 induced both I kappa B degradation and covalent modification of p65. CBP/p300-induced hyperacetylation as well as phosphorylation of p65 was implicated in ROS-mediated LTR activation. The results of our study showed that ROS-induced HIV LTR activation involves immediate early NF-kappa B activation at the post-translational level. (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码:180 / 185
页数:6
相关论文
共 50 条
  • [41] Particle-induced expression of SF20/IL25 is mediated by reactive oxygen species and NF-κB in alveolar macrophages
    Kim, Ji-Hye
    Jang, An-Soo
    Shin, Eun Kyong
    Kang, Chun-Mi
    Seok, Jung
    Lee, Eun Hee
    Kim, Myung Ok
    Park, Sung Woo
    Uh, SooTaek
    Park, Choon-Sik
    MOLECULAR & CELLULAR TOXICOLOGY, 2010, 6 (03) : 305 - 312
  • [42] HA14-1 sensitizes TNF-α-induced apoptosis via inhibition of the NF-κB signaling pathway: Involvement of reactive oxygen species and JNK
    Moon, Dong-Oh
    Kim, Mun-Ock
    Kang, Sang-Hyuck
    Choi, Yung Hyun
    Park, Sung Yong
    Kim, Gi-Young
    CANCER LETTERS, 2010, 292 (01) : 111 - 118
  • [43] Reactive oxygen species activate NFκB (p65) and p53 and induce apoptosis in RVFV infected liver cells
    Narayanan, Aarthi
    Amaya, Moushimi
    Voss, Kelsey
    Chung, Myung
    Benedict, Ashwini
    Sampey, Gavin
    Kehn-Hall, Kylene
    Luchini, Alessandra
    Liotta, Lance
    Bailey, Charles
    Kumar, Ajit
    Bavari, Sina
    Hakami, Ramin M.
    Kashanchi, Fatah
    VIROLOGY, 2014, 449 : 270 - 286
  • [44] Anesthetic Propofol Reduces Endotoxic Inflammation by Inhibiting Reactive Oxygen Species-regulated Akt/IKKβ/NF-κB Signaling
    Hsing, Chung-Hsi
    Lin, Ming-Chung
    Choi, Pui-Ching
    Huang, Wei-Ching
    Kai, Jui-In
    Tsai, Cheng-Chieh
    Cheng, Yi-Lin
    Hsieh, Chia-Yuan
    Wang, Chi-Yun
    Chang, Yu-Ping
    Chen, Yu-Hong
    Chen, Chia-Ling
    Lin, Chiou-Feng
    PLOS ONE, 2011, 6 (03):
  • [45] Reactive oxygen species-dependent Toll/NF-κB activation in the Drosophila hematopoietic niche confers resistance to wasp parasitism
    Louradour, Isabelle
    Sharma, Anurag
    Morin-Poulard, Ismael
    Letourneau, Manon
    Vincent, Alain
    Crozatier, Michele
    Vanzo, Nathalie
    ELIFE, 2017, 6
  • [46] Constitutive NF-κB activation and tumor-growth promotion by Romo1-mediated reactive oxygen species production
    Chung, Jin Sil
    Lee, Sora
    Yoo, Young Do
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2014, 450 (04) : 1656 - 1661
  • [47] Antioxidants tiron and N-acetyl-L-cysteine differentially mediate apoptosis in melanoma cells via a reactive oxygen species-independent NF-κB pathway
    Yang, Jinming
    Su, Yingjun
    Richmond, Ann
    FREE RADICAL BIOLOGY AND MEDICINE, 2007, 42 (09) : 1369 - 1380
  • [48] Eupatilin Inhibits Reactive Oxygen Species Generation via Akt/NF-κB/MAPK Signaling Pathways in Particulate Matter-Exposed Human Bronchial Epithelial Cells
    Lee, Dong Chang
    Oh, Jeong-Min
    Choi, Hyunsu
    Kim, Sung Won
    Kim, Soo Whan
    Kim, Byung Guk
    Cho, Jin Hee
    Lee, Joohyung
    Kim, Ji-Sun
    TOXICS, 2021, 9 (02) : 1 - 11
  • [49] Oxidative-nitrosative stress and post-translational protein modifications:: Implications to lung structure-function relations -: Arginase modulates NF-κB activity via a nitric oxide-dependent mechanism
    Ckless, Karina
    van der Vliet, Albert
    Janssen-Heininger, Yvonne
    AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2007, 36 (06) : 645 - 653
  • [50] Temporal Dynamics of Reactive Oxygen and Nitrogen Species and NF-κB Activation During Acute and Chronic T Cell-Driven Inflammation
    Schwenck, Johannes
    Mehling, Roman
    Thaiss, Wolfgang M.
    Kramer, Daniela
    Menendez, Irene Gonzalez
    Oez, Hasan Halit
    Hartl, Dominik
    Schulze-Osthoff, Klaus
    Hailfinger, Stephan
    Ghoreschi, Kamran
    Quintanilla-Martinez, Leticia
    Carlsen, Harald
    Roecken, Martin
    Pichler, Bernd J.
    Kneilling, Manfred
    MOLECULAR IMAGING AND BIOLOGY, 2020, 22 (03) : 504 - 514