Expression of functional interleukin-12 from mouse in transgenic tomato plants

被引：42

作者：

Gutiérrez-Ortega, A

Sandoval-Montes, C

Olivera-Flores, TD

Santos-Argumedo, L

Gómez-Lim, MA

机构：

[1] IPN, Ctr Invest & Estudios Avanzados, Dept Ingn Genet Plantas, Guanajuato 36500, Mexico

[2] Univ Nacl Autonoma Mexico, Fac Quim, Conjunto E, Lab 116, Mexico City 04510, DF, Mexico

来源：

TRANSGENIC RESEARCH | 2005年 / 14卷 / 06期

关键词：

interferon gamma; interleukin-12; recombinant proteins; tomato; transgenic plants;

D O I：

10.1007/s11248-005-1464-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Transgenic plants have been employed successfully as a low-cost system for the production of therapeutically valuable proteins, including antibodies, antigens and hormones. Here, we report the expression of a cytokine with immunomodulatory function, mouse interleukin-12 (IL-12), in transgenic tomato plants. Single-chain mouse IL-12 driven by the CaMV 35S promoter, accumulates to high levels in leaves and fruits (up to 7.3 and 3.4 mu g per gram of fresh weight, respectively). Mouse IL-12 expressed in tomato displays biological activity in vitro, as determined by interferon-gamma (IFN-gamma) secretion by T cells. Possible uses of this plant-based cytokine involving mucosal delivery are discussed.

引用

页码：877 / 885

页数：9

共 47 条

[1] Analysis of the N-glycosylation of recombinant glycoproteins produced in transgenic plants [J].