Regional assignment of EST sequences on human chromosome 13

被引：9

作者：

Hawthorn, LA ^{[1
]}

Cowell, JK ^{[1
]}

机构：

[1] CLEVELAND CLIN FDN,RES INST,DEPT NEUROSCI NC30,CLEVELAND,OH 44195

来源：

CYTOGENETICS AND CELL GENETICS | 1996年 / 72卷 / 01期

关键词：

D O I：

10.1159/000134166

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have used a panel of somatic cell hybrids carrying structural rearrangements of human chromosome 13 to regionally localise 10 expressed sequence tags (ESTs) from this chromosome. Three of these genes were not present in the somatic cell hybrid PGME, which contains chromosome 13 as its major human component. Using a panel of somatic cell hybrids, two of these genes were localised to chromosome 8 and one to chromosome 16. Using the PCR primers as published, it was impossible to map one EST; consequently, a YAC containing this gene was isolated, and fluorescence in situ hybridisation was used to map the gene to 13q14. The distribution of the remaining six chromosome 13-specific ESTs was nonrandom; one mapped to 13q14 and the remaining five to 13q12, with three mapping within the proximal portion and two mapping within the distal portion of this region.

引用

页码：72 / 77

页数：6

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