Development of an electroanalytical method to control quality in fish samples based on an edge plane pyrolytic graphite electrode. Simultaneous determination of hypoxanthine, xanthine and uric acid

被引：47

作者：

Dario Pierini, Gaston ^{[1
]}

Noel Robledo, Sebastian ^{[2
]}

Alicia Zon, Maria ^{[1
]}

Susana Di Nezio, Maria ^{[3
]}

Marcelo Granero, Adrian ^{[1
]}

Fernandez, Hector ^{[1
]}

机构：

[1] Univ Nacl Rio Cuarto, Fac Ciencias Exactas Fis Quim & Nat, Dept Quim, Grp Electroanalit GEANA, Agencia Postal 3, RA-5800 Rio Cuarto, Argentina

[2] Univ Nacl Rio Cuarto, Fac Ingn, Dept Tecnol Quim, Grp Electroanalit GEANA, Agencia Postal 3, RA-5800 Rio Cuarto, Argentina

[3] Univ Nacl Sur, Dept Quim, INQUISUR, Bahia Blanca, Buenos Aires, Argentina

来源：

MICROCHEMICAL JOURNAL | 2018年 / 138卷

关键词：

Hypoxanthine; Xanthine; Uric acid; Experimental design; Edge plane pyrolytic graphite electrode; Fish samples; GLASSY-CARBON ELECTRODES; ELECTROCHEMICAL PRETREATMENT; CHEMOMETRIC TOOLS; PASTE ELECTRODE; SENSOR; OPTIMIZATION; BIOSENSOR; NANOTUBES; REMOVAL; INOSINE;

D O I：

10.1016/j.microc.2017.12.025

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple, rapid and non-expensive method is proposed to determine hypoxanthine (Hx), xanthine (Xa) and uric acid (UA) in untreated fish samples based on an edge plane pyrolytic graphite electrode (EPPGE). The square wave voltammetry was the electrochemical technique used. In addition, the experimental design and multi-response assays were used to optimize the pretreatment of the working electrode. Therefore, the EPPGE is used in the simultaneous determination of these analytes for the first time. The optimal accumulation conditions of the analytes on the surface of the working electrode were obtained through a composite central design. The linear range was from 0.1 to 50 M for Hx and Xa, and from 0.1 to 25.0 mu M for UA. The limits of detection (LOD) were 0.08, 0.06 and 0.03 mu M for Hx, Xa and UA, respectively. Recovery assays were made in order to validate the proposed method. Recovery percentages were between 90 and 110%. Therefore, the method is a good, simple and fast option for the simultaneous determination of Hx, Xa and UA in fish samples, allowing to perform a control quality of samples. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：58 / 64

页数：7

共 44 条

[1] Graphene-titanium dioxide nanocomposite based hypoxanthine sensor for assessment of meat freshness [J].

Albelda, Jasmine A. V. ;

Uzunoglu, Aytekin ;

Santos, Gil Nonato C. ;

Stanciu, Lia A. .

BIOSENSORS & BIOELECTRONICS, 2017, 89 :518-524

[2] ELECTROCHEMICAL PRETREATMENT OF CARBON ELECTRODES AS A FUNCTION OF POTENTIAL, PH, AND TIME [J].

BEILBY, AL ;

SASAKI, TA ;

STERN, HM .

ANALYTICAL CHEMISTRY, 1995, 67 (05) :976-980

[3] Response surface methodology (RSM) as a tool for optimization in analytical chemistry [J].

Bezerra, Marcos Almeida ;

Santelli, Ricardo Erthal ;

Oliveira, Eliane Padua ;

Villar, Leonardo Silveira ;

Escaleira, Luciane Amlia .

TALANTA, 2008, 76 (05) :965-977

[4] Comparison of capillary electrophoretic and liquid chromatographic determination of hypoxanthine and xanthine for the diagnosis of xanthinuria [J].

Bory, C ;

Chantin, C ;

Boulieu, R .

JOURNAL OF CHROMATOGRAPHY A, 1996, 730 (1-2) :329-331

[5] Development of a bienzymic graphite-Teflon composite electrode for the determination of hypoxanthine in fish [J].

Cayuela, G ;

Peña, N ;

Reviejo, AJ ;

Pingarrón, JM .

ANALYST, 1998, 123 (02) :371-377

[6] Selective removal of ATP degradation products from food matrices II: Rapid screening of hypoxanthine and inosine by molecularly imprinted matrix solid-phase dispersion for evaluation of fish freshness [J].

Cela-Perez, M. C. ;

Barbosa-Pereira, L. ;

Vecino, X. ;

Perez-Ameneiro, M. ;

Lasagabaster Latorre, Aurora ;

Lopez-Vilarino, J. M. ;

Gonzalez Rodriguez, M. V. ;

Moldes, A. B. ;

Cruz, J. M. .

TALANTA, 2015, 135 :58-66

[7] Quantification of uric acid, xanthine and hypoxanthine in human serum by HPLC for pharmacodynamic studies [J].

Cooper, Nancy ;

Khosravan, Reza ;

Erdmann, Carol ;

Fiene, John ;

Lee, Jean W. .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2006, 837 (1-2) :1-10

[8]

DERRINGER G, 1980, J QUAL TECHNOL, V12, P214, DOI 10.1080/00224065.1980.11980968

[9] Electrochemical biosensor based on REGO/Fe3O4 bionanocomposite interface for xanthine detection in fish sample [J].

Dervisevic, Muamer ;

Custiuc, Esma ;

Cevik, Emre ;

Durmus, Zehra ;

Senel, Mehmet ;

Durmus, Ali .

FOOD CONTROL, 2015, 57 :402-410

[10] Construction of novel xanthine biosensor by using polymeric mediator/MWCNT nanocomposite layer for fish freshness detection [J].

Dervisevic, Muamer ;

Custiuc, Esma ;

Cevik, Emre ;

Senel, Mehmet .

FOOD CHEMISTRY, 2015, 181 :277-283

← 1 2 3 4 5 →