Mfn2 modulates the UPR and mitochondrial function via repression of PERK

被引:361
作者
Pablo Munoz, Juan [1 ,2 ,3 ]
Ivanova, Saska [1 ,2 ,3 ]
Sanchez-Wandelmer, Jana [1 ,2 ,3 ]
Martinez-Cristobal, Paula [1 ,2 ,3 ]
Noguera, Eduard [1 ,2 ,3 ]
Sancho, Ana [1 ,2 ,3 ]
Diaz-Ramos, Angels [1 ,2 ,3 ]
Isabel Hernandez-Alvarez, Maria [1 ,2 ,3 ]
Sebastian, David [1 ,2 ,3 ]
Mauvezin, Caroline [1 ,2 ,3 ]
Palacin, Manuel [1 ,2 ]
Zorzano, Antonio [1 ,2 ,3 ]
机构
[1] Inst Res Biomed IRB Barcelona, Barcelona 08028, Spain
[2] Univ Barcelona, Fac Biol, Dept Bioquim & Biol Mol, Barcelona, Spain
[3] Inst Salud Carlos III, CIBER Diabet & Enfermedades Metab Asociadas CIBER, Barcelona, Spain
关键词
cell death and autophagy; cell metabolism; mitochondria; ENDOPLASMIC-RETICULUM STRESS; UNFOLDED PROTEIN RESPONSE; MUSCLE-CELL APOPTOSIS; MITOFUSIN; ER STRESS; OXIDATIVE STRESS; GENE-EXPRESSION; CANCER-CELLS; CA2+ PUMP; FUSION;
D O I
10.1038/emboj.2013.168
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mitofusin 2 (Mfn2) is a key protein in mitochondrial fusion and it participates in the bridging of mitochondria to the endoplasmic reticulum (ER). Recent data indicate that Mfn2 ablation leads to ER stress. Here we report on the mechanisms by which Mfn2 modulates cellular responses to ER stress. Induction of ER stress in Mfn2-deficient cells caused massive ER expansion and excessive activation of all three Unfolded Protein Response (UPR) branches (PERK, XBP-1, and ATF6). In spite of an enhanced UPR, these cells showed reduced activation of apoptosis and autophagy during ER stress. Silencing of PERK increased the apoptosis of Mfn2-ablated cells in response to ER stress. XBP-1 loss-of-function ameliorated autophagic activity of these cells upon ER stress. Mfn2 physically interacts with PERK, and Mfn2-ablated cells showed sustained activation of this protein kinase under basal conditions. Unexpectedly, PERK silencing in these cells reduced ROS production, normalized mitochondrial calcium, and improved mitochondrial morphology. In summary, our data indicate that Mfn2 is an upstream modulator of PERK. Furthermore, Mfn2 loss-of-function reveals that PERK is a key regulator of mitochondrial morphology and function.
引用
收藏
页码:2348 / 2361
页数:14
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