Comparative Performance of Microbial Lipases Immobilized on Magnetic Polysiloxane Polyvinyl Alcohol Particles

被引：11

作者：

Bruno, Laura Maria ^{[1
]}

de Lima Filho, Jose Luiz ^{[2
]}

de Castro, Heizir Ferreira ^{[3
]}

机构：

[1] Embrapa Agroind Trop, BR-60511110 Fortaleza, Ceara, Brazil

[2] Univ Fed Pernambuco, Dept Bioquim, BR-50670420 Recife, PE, Brazil

[3] Univ Sao Paulo, Escola Engn Lorena, BR-12600970 Lorena, SP, Brazil

来源：

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY | 2008年 / 51卷 / 05期

关键词：

Candida lipase; Mucor lipase; hybrid matrix; immobilization;

D O I：

10.1590/S1516-89132008000500003

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Microbial lipase from Mucor miehei and Candida rugosa were immobilized by covalent binding onto magnetized polysiloxane polyvinyl alcohol particles (POS-PVA). The resulting immobilized derivatives were evaluated in aqueous solution (olive oil hydrolysis) and organic solvent (butyl butyrate synthesis). Higher catalytic activities were found when the coupling procedure was made with M. miehei lipase. Immobilized M. miehei lipase also showed a better operational stability and a higher half-life than C. rugosa lipase after the successive batches of esterification. The performance of C. rugosa immobilized derivative was constrained by the low lipase loading used in the immobilizing step. Further information regarding the both immobilized derivatives was also obtained through chemical composition (FTIR).

引用

页码：889 / 896

页数：8

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