Molecular characterization of Prototheca strains isolated from bovine mastitis

被引：21

作者：

Aouay, A. ^{[1
]}

Coppee, F. ^{[1
]}

Cloet, S. ^{[1
]}

Cuvelier, P. ^{[2
]}

Belayew, A. ^{[1
]}

Lagneau, P. -E. ^{[2
]}

Mullender, C. ^{[2
]}

机构：

[1] Univ Mons, Mol Biol Lab, B-7000 Mons, Belgium

[2] Reg Assoc Hlth & Anim Identificat, B-5590 Ciney, Belgium

来源：

JOURNAL DE MYCOLOGIE MEDICALE | 2008年 / 18卷 / 04期

关键词：

Algae; Prototheca; Bovine; Mastitis; PCR-RFLP;

D O I：

10.1016/j.mycmed.2008.10.002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Objective. - Prototheca zopfii are colourless algae that can cause mastitis in dairy cattle. Based on sequence analysis of the 18S rRNA gene, three genotypes are described. Material and method. - To differentiate them, a recent method of diagnostic was proposed based on rDNA amplification by Polymerase Chain Reaction (PCR) and Restriction Fragments Length Polymorphism analysis (RFLP). A total of 30 strains isolated from mastitis cases becoming of various farms were investigated. Results. - The molecular study allowed the identification of 27 strains belonging to genotype II, three to genotype III and no one to genotype I. Conclusion. - The simple PCR/RFLP system enabled the rapid, accurate and easily performed identification of genotypes of the species "zopfii". (C) 2008 Elsevier Masson SAS. All rights reserved.

引用

页码：224 / 227

页数：4

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