Application of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Discrimination of Laboratory-Derived Antibiotic-Resistant Bacteria

被引：9

作者：

Muroi, Masashi ^{[1
]}

Shima, Keisuke ^{[2
]}

Igarashi, Masayuki ^{[3
]}

Nakagawa, Yasuyoshi ^{[4
]}

Tanamoto, Ken-ichi ^{[1
]}

机构：

[1] Musashino Univ, Pharmaceut Sci Res Inst, Tokyo 2028585, Japan

[2] Shimadzu Co Ltd, Analyt & Measuring Instruments Div, Nakagyo Ku, Kyoto 6048511, Japan

[3] Microbial Chem Res Fdn, Shinagawa, Tokyo 1410021, Japan

[4] Natl Inst Technol & Evaluat, Resource Collect Div, Kisarazu, Chiba 2920818, Japan

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2012年 / 35卷 / 10期

关键词：

mass spectrometry; antibiotics; bacteria; STAPHYLOCOCCUS-AUREUS; ESCHERICHIA-COLI; TETRACYCLINE RESISTANCE; INTACT MICROORGANISMS; MUTANTS RESISTANT; RIBOSOMAL-RNA; RPSL GENE; MALDI; IDENTIFICATION; PROTEINS;

D O I：

10.1248/bpb.b12-00465

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The ability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to discriminate laboratory-derived antibiotic-resistant bacterial strains of known genetic origin was examined. A computer-based cluster analysis of spectral data successfully discriminated the majority of single- as well as multiple-antibiotic-resistant Escherichia coli strains examined. Cluster analysis of Staphylococcus aureus strains with different levels of novobiocin resistance showed that as the degree of resistance increased similarity to the wild-type strain decreased. These results demonstrate that MALDI-TOF MS is capable of discriminating antibiotic-resistant bacterial strains and may have potential for differentiating bacterial strains with varying degrees of antibiotic-resistance.

引用

页码：1841 / 1845

页数：5

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