Urocortin 2 acts centrally to delay gastric emptying through sympathetic pathways while CRF and urocortin 1 inhibitory actions are vagal dependent in rats

被引:52
作者
Czimmer, J
Million, M
Taché, Y
机构
[1] VA Greater Los Angeles Healthcare Syst, Los Angeles, CA 90073 USA
[2] Univ Calif Los Angeles, CURE, Digest Dis Res Ctr, Los Angeles, CA USA
[3] Univ Calif Los Angeles, Ctr Neurovisceral Sci & Womens Hlth, Div Digest Dis, Dept Med, Los Angeles, CA USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | 2006年 / 290卷 / 03期
关键词
adrenergic receptors; vagus; corticotropin; releasing hormone receptor;
D O I
10.1152/ajpgi.00289.2005
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Urocortin 2 acts centrally to delay gastric emptying through sympathetic pathways while CRF and urocortin 1 inhibitory actions are vagal dependent in rats. Am J Physiol Gastrointest Liver Physiol 290: G511-G518, 2006. First published Octobert 13, 2005; doi: 10.1152/ajpgi.00289.2005.-We characterized the influence of the selective corticotropin-releasing factor 2 (CRF2) receptor agonist human urocortin 2 (Ucn 2), injected intracisternally, on gastric emptying and its mechanism of action compared with intracisternal CRF or urocortin (Ucn 1) in conscious rats. The methylcellulose phenol red solution was gavaged 20 min after peptide injection, and gastric emptying was measured 20 min later. The intracisternal injection of Ucn 2 (0.1 and 1 mu g) and Ucn 1 (1 mu g) decreased gastric emptying to 37.8 +/- 6.9%, 23.1 +/- 8.6%, and 21.6 +/- 5.9%, respectively, compared with 58.4 +/- 3.8% after intracisternal vehicle. At lower doses, Ucn 2 (0.03 mu g) and Ucn 1 (0.1 mu g) had no effect. The CRF2 antagonist astressin(2)-B (3 mu g ic) antagonized intracisternal Ucn 2 (0.1 mu g) and CRF (0.3 mu g)-induced inhibition of gastric emptying. Vagotomy enhanced intracisternal Ucn 2 (0.1 or 1 mu g)-induced inhibition of gastric emptying compared with sham-operated group, whereas it blocked intracisternal CRF (1 mu g) inhibitory action (45.5 +/- 8.4% vs. 9.7 +/- 9.7%). Sympathetic blockade by bretylium prevented intracisternal and intracerebroventricular Ucn 2-induced delayed gastric emptying, whereas it did not influence intravenous Ucn 2-, intracisternal CRF-, and intracisternal Ucn 1-induced inhibition of gastric emptying. Prazosin abolished the intracisternal Ucn 2 inhibitory effect, whereas yohimbine and propranolol did not. None of the pretreatments modified basal gastric emptying. These data indicate that intracisternal Ucn 2 induced a central CRF2-mediated inhibition of gastric emptying involving sympathetic alpha(1)-adrenergic mechanisms independent from the vagus contrasting with the vagal-dependent inhibitory actions of CRF and Ucn 1.
引用
收藏
页码:G511 / G518
页数:8
相关论文
共 51 条
  • [1] ABRAHAMSSON H, 1984, SCAND J GASTROENTERO, V19, P83
  • [2] CRF and CRF receptors: Role in stress responsivity and other behaviors
    Bale, TL
    Vale, WW
    [J]. ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 2004, 44 : 525 - 557
  • [3] Boeckxstaens GE, 1999, NEUROGASTROENT MOTIL, V11, P467
  • [4] ADRENERGIC, CHOLINERGIC AND VIP-ERGIC INFLUENCE ON GASTRIC PHASIC MOTILITY IN THE RAT
    BOJO, L
    CASSUTO, J
    NELLGARD, P
    JONSSON, A
    [J]. ACTA PHYSIOLOGICA SCANDINAVICA, 1994, 150 (01): : 67 - 73
  • [5] Pharmacological and chemical properties of astressin, antisauvagine-30 and α-helCRF:: significance for behavioral experiments
    Brauns, O
    Liepold, T
    Radulovic, J
    Spiess, J
    [J]. NEUROPHARMACOLOGY, 2001, 41 (04) : 507 - 516
  • [6] PITUITARY-ADRENAL AND VAGUS MODULATION OF SAUVAGINE-INDUCED AND CRF-INDUCED INHIBITION OF GASTRIC-EMPTYING IN RATS
    BROCCARDO, M
    IMPROTA, G
    [J]. EUROPEAN JOURNAL OF PHARMACOLOGY, 1990, 182 (02) : 357 - 362
  • [7] STUDIES ON THE MODE OF ACTION OF BRETYLIUM AND GUANETHIDINE IN POST-GANGLIONIC SYMPATHETIC-NERVE FIBERS
    BROCK, JA
    CUNNANE, TC
    [J]. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 1988, 338 (05) : 504 - 509
  • [8] BURNSTOCK G, 2004, STRUCTURAL CHEM ORG, P1
  • [9] A soluble mouse brain splice variant of type 2α corticotropin-releasing factor (CRF) receptor binds ligands and modulates their activity
    Chen, AM
    Perrin, MH
    DiGruccio, MR
    Vaughan, JM
    Brar, BK
    Arias, CM
    Lewis, KA
    Rivier, JE
    Sawchenko, PE
    Valet, WW
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (07) : 2620 - 2625
  • [10] Intracisternal urocortin inhibits vagally stimulated gastric motility in rats:: role of CRF2
    Chen, CY
    Million, M
    Adelson, DW
    Martínez, V
    Rivier, J
    Taché, Y
    [J]. BRITISH JOURNAL OF PHARMACOLOGY, 2002, 136 (02) : 237 - 247