Constitutive expression of clathrin hub hinders elicitor-induced clathrin-mediated endocytosis and defense gene expression in plant cells

被引:18
作者
Adam, T. [1 ]
Bouhidel, K. [1 ]
Der, C. [1 ]
Robert, F. [1 ]
Najid, A. [1 ]
Simon-Plas, F. [1 ]
Leborgne-Castel, N. [1 ]
机构
[1] Univ Bourgogne, UMR Agroecol 1347, AgroSup, INRA,Pole Interact Plantes Microorganismes,CNRS E, F-21065 Dijon, France
关键词
Bright Yellow-2; Clathrin; Cryptogein; Endocytosis; Cell signaling; AUXIN EFFLUX CARRIERS; TOBACCO BY-2 CELLS; PLASMA-MEMBRANE; DEPENDENT ENDOCYTOSIS; ARABIDOPSIS; PROTEINS; RECEPTOR; PHRAGMOPLAST; TRAFFICKING; PATHWAYS;
D O I
10.1016/j.febslet.2012.06.053
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Endocytosis has been recently implicated in the signaling network associated with the recognition of microbes by plants. In a previous study, we showed that the elicitor cryptogein was able to induce clathrin-mediated endocytosis (CME) in tobacco suspension cells. Herein, we investigate further the induced CME by means of a GFP-tagged clathrin light chain and a CME inhibitor, the hub domain of clathrin heavy chain. Hub constitutive expression does affect neither cell growth nor constitutive endocytosis but abolishes cryptogein-induced CME. Such an inhibition has no impact on early events in the cryptogein signaling pathway but reduces the expression of defense-associated genes. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:3293 / 3298
页数:6
相关论文
共 31 条
[1]   Transcriptome analysis of tobacco BY-2 cells elicited by cryptogein reveals new potential actors of calcium-dependent and calcium-independent plant defense pathways [J].
Amelot, Nicolas ;
de Borne, Francois Dorlhac ;
Clemente, Helene San ;
Mazars, Christian ;
Grima-Pettenati, Jacqueline ;
Briere, Christian .
CELL CALCIUM, 2012, 51 (02) :117-130
[2]   Monoubiquitin-dependent endocytosis of the IRON-REGULATED TRANSPORTER 1 (IRT1) transporter controls iron uptake in plants [J].
Barberon, Marie ;
Zelazny, Enric ;
Robert, Stephanie ;
Conejero, Genevieve ;
Curie, Cathy ;
Friml, Jiri ;
Vert, Gregory .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (32) :E450-E458
[3]   Clathrin-coated pits: vive la difference? [J].
Benmerah, Alexandre ;
Lamaze, Christophe .
TRAFFIC, 2007, 8 (08) :970-982
[4]   Clathrin-mediated endocytosis: the gateway into plant cells [J].
Chen, Xu ;
Irani, Niloufer G. ;
Friml, Jiri .
CURRENT OPINION IN PLANT BIOLOGY, 2011, 14 (06) :674-682
[5]   Clathrin-mediated constitutive endocytosis of PIN auxin efflux carriers in Arabidopsis [J].
Dhonukshe, Pankaj ;
Aniento, Fernando ;
Hwang, Inhwan ;
Robinson, David G. ;
Mravec, Jozef ;
Stierhof, York-Dieter ;
Friml, Jiri .
CURRENT BIOLOGY, 2007, 17 (06) :520-527
[6]   Imaging of plant dynamin-related proteins and clathrin around the plasma membrane by variable incidence angle fluorescence microscopy [J].
Fujimoto, Masaru ;
Arimura, Shin-ichi ;
Nakazono, Mikio ;
Tsutsumi, Nobuhiro .
PLANT BIOTECHNOLOGY, 2007, 24 (05) :449-455
[7]   Polar-localized NPH3-like proteins regulate polarity and endocytosis of PIN-FORMED auxin efflux carriers [J].
Furutani, Masahiko ;
Sakamoto, Norihito ;
Yoshida, Shuhei ;
Kajiwara, Takahito ;
Robert, Helene S. ;
Friml, Jiri ;
Tasaka, Masao .
DEVELOPMENT, 2011, 138 (10) :2069-2078
[8]   Spatial control of coated-pit dynamics in living cells [J].
Gaidarov, I ;
Santini, F ;
Warren, RA ;
Keen, JH .
NATURE CELL BIOLOGY, 1999, 1 (01) :1-7
[9]   Early signaling events induced by elicitors of plant Defenses [J].
Garcia-Brugger, Angela ;
Lamotte, Olivier ;
Vandelle, Elodie ;
Bourque, Stephane ;
Lecourieux, David ;
Poinssot, Benoit ;
Wendehenne, David ;
Pugin, Alain .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2006, 19 (07) :711-724
[10]   Dynamic behavior of clathrin in Arabidopsis thaliana unveiled by live imaging [J].
Ito, Emi ;
Fujimoto, Masaru ;
Ebine, Kazuo ;
Uemura, Tomohiro ;
Ueda, Takashi ;
Nakano, Akihiko .
PLANT JOURNAL, 2012, 69 (02) :204-216