Constitutive expression of clathrin hub hinders elicitor-induced clathrin-mediated endocytosis and defense gene expression in plant cells

被引：18
作者：
Adam, T. [1 ]
Bouhidel, K. [1 ]
Der, C. [1 ]
Robert, F. [1 ]
Najid, A. [1 ]
Simon-Plas, F. [1 ]
Leborgne-Castel, N. [1 ]
机构：
[1] Univ Bourgogne, UMR Agroecol 1347, AgroSup, INRA,Pole Interact Plantes Microorganismes,CNRS E, F-21065 Dijon, France
来源：
FEBS LETTERS | 2012年 / 586卷 / 19期
关键词：
Bright Yellow-2; Clathrin; Cryptogein; Endocytosis; Cell signaling; AUXIN EFFLUX CARRIERS; TOBACCO BY-2 CELLS; PLASMA-MEMBRANE; DEPENDENT ENDOCYTOSIS; ARABIDOPSIS; PROTEINS; RECEPTOR; PHRAGMOPLAST; TRAFFICKING; PATHWAYS;
D O I：
10.1016/j.febslet.2012.06.053
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Endocytosis has been recently implicated in the signaling network associated with the recognition of microbes by plants. In a previous study, we showed that the elicitor cryptogein was able to induce clathrin-mediated endocytosis (CME) in tobacco suspension cells. Herein, we investigate further the induced CME by means of a GFP-tagged clathrin light chain and a CME inhibitor, the hub domain of clathrin heavy chain. Hub constitutive expression does affect neither cell growth nor constitutive endocytosis but abolishes cryptogein-induced CME. Such an inhibition has no impact on early events in the cryptogein signaling pathway but reduces the expression of defense-associated genes. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：3293 / 3298
页数：6
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