Cajan leaf combined with bone marrow-derived mesenchymal stem cells for the treatment of osteonecrosis of the femoral head

被引：11

作者：

Shi, Da ^{[1
]}

Sun, Yindi ^{[1
]}

Yin, Jichao ^{[1
]}

Fan, Xiaochen ^{[1
]}

Duan, Honghao ^{[1
]}

Liu, Na ^{[1
]}

He, Wei ^{[2
]}

机构：

[1] Xi An Jiao Tong Univ, Coll Med, Hong Hui Hosp, Dept Orthoped Tradit Chinese Med, Xian 710054, Shaanxi, Peoples R China

[2] Guangzhou Univ Chinese Med, Affiliated Hosp 1, Dept Orthoped, Guangzhou 510405, Guangdong, Peoples R China

来源：

EXPERIMENTAL AND THERAPEUTIC MEDICINE | 2014年 / 7卷 / 06期

关键词：

cajan leaf; mesenchymal stem cell; osteonecrosis of the femoral head; treatment; EARLY-STAGE OSTEONECROSIS; DECOMPRESSION; PATHOGENESIS; IMPLANTATION; POLYMORPHISM; ASSOCIATION; GENE;

D O I：

10.3892/etm.2014.1622

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The aim of the present study was to observe the curative effect of traditional Chinese cajan leaves, combined with administration of bone marrow-derived mesenchymal stem cells (BMSCs), on osteonecrosis of the femoral head (ONFH) in rats and to investigate the underlying mechanisms. A total of 40 rat ONFH models were established, through liquid nitrogen freezing and were subsequently divided into groups: A, control; B, treated with cajan leaf; C, treated with BMSCs and D, treated with cajan leaf combined with BMSCs. Samples were obtained 30 days following treatment, and immunohistochemical staining of vascular endothelial growth factor (VEGF) and image analysis were performed. Chondrocytes and vascular endothelial cells were stained as a result of immunohistochemical staining and group D exhibited markedly deeper staining, and a significantly larger number of stained cells, compared with group A. Thus, in the present study, cajan leaf combined with BMSCs was shown to promote VEGF expression and improve ONFH repair.

引用

页码：1471 / 1475

页数：5

共 23 条

[1]

Aaron RK., 2001, TECH ORTHOP, V16, P101

[2]

Assouline-Dayan Y, 2002, SEMIN ARTHRITIS RHEU, V32, P94, DOI 10.1053/sarh.2002.33724

[3] Osteonecrosis of the Femoral Head [J].

Babis, George C. ;

Sakellariou, Vasileios ;

Parvizi, Javad ;

Soucacos, Panayiotis .

ORTHOPEDICS, 2011, 34 (01) :39-48

[4] Long-term results of decompression and muscle-pedicle bone grafting for osteonecrosis of the femoral head [J].

Baksi, Durgapada P. ;

Pal, Ananda Kisor ;

Baksi, Debadyuti D. .

INTERNATIONAL ORTHOPAEDICS, 2009, 33 (01) :41-47

[5] Autologous bone marrow cell implantation in the treatment of non-traumatic osteonecrosis of the femoral head: Five year follow-up of a prospective controlled study [J].

Gangji, Valerie ;

De Maertelaer, Viviane ;

Hauzeur, Jean-Philippe .

BONE, 2011, 49 (05) :1005-1009

[6] Treatment of Osteonecrosis of the Femoral Head with VEGF165 Transgenic Bone Marrow Mesenchymal Stem Cells in Mongrel Dogs [J].

Hang, Donghua ;

Wang, Qiugen ;

Guo, Changan ;

Chen, Zhengrong ;

Yan, Zuoqin .

CELLS TISSUES ORGANS, 2012, 195 (06) :495-506

[7] Genetic association of angiogenesis- and hypoxia-related gene polymorphisms with osteonecrosis of the femoral head [J].

Hong, Jung Min ;

Kim, Tae-Ho ;

Kim, Hyun-Ju ;

Park, Eui-Kyun ;

Yang, Eun-Kyoung ;

Kim, Shin-Yoon .

EXPERIMENTAL AND MOLECULAR MEDICINE, 2010, 42 (05) :376-385

[8]

Ivankovich DA, 2001, TECH ORTHOP, V16, P66

[9]

Jones LC, 2003, CURR OPIN ORTHOP, V14, P12

[10] Lack of association of MTHFR gene polymorphisms with the risk of osteonecrosis of the femoral head in a Korean population [J].

Kim, Tae-Ho ;

Hong, Jung Min ;

Kim, Hyun-Ju ;

Park, Eui Kyun ;

Kim, Shin-Yoon .

MOLECULES AND CELLS, 2010, 29 (04) :343-348

← 1 2 3 →