Molecular Survey of Anaplasma Species in Small Ruminants Reveals the Presence of Novel Strains Closely Related to A. phagocytophilum in Tunisia

被引：68
作者：
Ben Said, Mourad [1 ]
Belkahia, Hanene [1 ,2 ]
Alberti, Alberto [3 ]
Zobba, Rosanna [3 ]
Bousrih, Maha [1 ]
Yahiaoui, Mouna [1 ]
Daaloul-Jedidi, Monia [1 ]
Mamlouk, Aymen [1 ]
Gharbi, Mohamed [4 ]
Messadi, Lilia [1 ]
机构：
[1] Univ La Manouba, Lab Microbiol, Ecole Natl Med Vet, Sidi Thabet, Tunisia
[2] Univ Carthage, Fac Sci Bizerte, Jarzouna, Tunisia
[3] Univ Sassari, Dipartimento Med Vet, I-07100 Sassari, Italy
[4] Univ La Manouba, Lab Parasitol, Ecole Natl Med Vet, Sidi Thabet, Tunisia
来源：
VECTOR-BORNE AND ZOONOTIC DISEASES | 2015年 / 15卷 / 10期
关键词：
Anaplasma ovis; A; phagocytophilum and related species; Small ruminants; Molecular prevalence; 16S rRNA and msp4 genes; Tunisia; EHRLICHIA-EQUI; IXODID TICKS; OVIS; IDENTIFICATION; RICKETTSIALES; INFECTION; MARGINALE; HOKKAIDO; AGENT; SHEEP;
D O I：
10.1089/vbz.2015.1796
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
A survey of Anaplasma species in small ruminants is still lacking in North African countries. In this study, the presence of A. phagocytophilum, A. phagocytophilum-related species, and A. ovis was investigated in a total of 563 healthy small ruminants (303 goats and 260 sheep), from 25 randomly selected flocks sampled in Tunisia. Anaplasma spp. and A. ovis overall infection rates were 95.0% and 93.8% in sheep and 69.6% and 65.3% in goats, respectively. A. phagocytophilum was not detected in any of tested animals. A total of 20 sheep (7.7%) and 144 goats (47.5%) were infected by Anaplasma strains genetically related to A. phagocytophilum. Both in sheep and goats A. ovis prevalence was higher in adults (2 years) than in young (<2 years) subjects (p=0.001 and 0.002 for goats and sheep, respectively). In sheep, A. ovis prevalence was higher in ewes with respect to rams (p=0.010). The A. ovis infection rate was significantly lower in goats of the local breed (p=0.049) and it was higher in goats infested by ticks than in not infested animals (p=0.005). Genetic analysis of the msp4 gene of A. ovis indicated the presence of strains shared by Tunisian sheep and goats. Sequence analysis and phylogenetic studies on the basis of the 16S rRNA gene provided evidence for the circulation of at least two different potentially novel species genetically related to A. phagocytophilum in Tunisian small ruminants. These findings cause concern about specificity of serological tests used for detection of A. phagocytophilum in ruminants and provide additional information for elucidating pathogenesis and molecular epidemiology of A. phagocytophilum and related species.
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收藏
页码：580 / 590
页数：11
相关论文
共 52 条
[11]  
Bouattour A, 2002, Arch Inst Pasteur Tunis, V79, P43
[12]  
Bouattour A., 1999, Parassitologia (Rome), V41, P5
[13]   Development of a Real-Time PCR Assay for Detection and Quantification of Anaplasma ovis Infection [J].
Chi, Q. ;
Liu, Z. ;
Li, Y. ;
Yang, J. ;
Chen, Z. ;
Yue, C. ;
Luo, J. ;
Yin, H. .
TRANSBOUNDARY AND EMERGING DISEASES, 2013, 60 :119-124
[14]   Human Anaplasmosis and Anaplasma ovis Variant [J].
Chochlakis, Dimosthenis ;
Ioannou, Ioannis ;
Tselentis, Yannis ;
Psaroulaki, Anna .
EMERGING INFECTIOUS DISEASES, 2010, 16 (06) :1031-1032
[15]  
Darghouth M A, 2004, Arch Inst Pasteur Tunis, V81, P21
[16]   Serologic and molecular characterization of Anaplasma species infection in farm animals and ticks from Sicily [J].
de la Fuente, J ;
Torina, A ;
Caracappa, S ;
Tumino, G ;
Furlá, R ;
Almazán, C ;
Kocan, KM .
VETERINARY PARASITOLOGY, 2005, 133 (04) :357-362
[17]  
de la Fuente J, 2007, VET MICROBIOL, V119, P375, DOI [10.1016/j.vetmic.2006.09.011, 10.1016/j.vetmic.2006.09.015]
[18]   Survey of Anaplasmataceae bacteria in sheep from Senegal [J].
Djiba, Mamadou Lamine ;
Mediannikov, Oleg ;
Mbengue, Mbaye ;
Thiongane, Yaya ;
Molez, Jean-Francois ;
Seck, Momar Talla ;
Fenollar, Florence ;
Raoult, Didier ;
Ndiaye, Mady .
TROPICAL ANIMAL HEALTH AND PRODUCTION, 2013, 45 (07) :1557-1561
[19]   Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales:: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and 'HGE agent' as subjective synonyms of Ehrlichia phagocytophila [J].
Dumler, JS ;
Barbet, AF ;
Bekker, CPJ ;
Dasch, GA ;
Palmer, GH ;
Ray, SC ;
Rikihisa, Y ;
Rurangirwa, FR .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2001, 51 :2145-2165
[20]  
Friedhoff K. T., 1997, Parassitologia (Rome), V39, P99
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