Molecular epidemiology of classical swine fever in Cuba

被引:0
作者
de Arce, HD
Núñez, JI
Ganges, L
Barreras, M
Frías, MT
Sobrino, F [1 ]
机构
[1] INIA, Ctr Invest Sanidad Anim, Madrid 28130, Spain
[2] Ctr Nacl Sanidad Agropecuaria, La Habana, Cuba
[3] UAM, CSIC, Ctr Biol Mol Severo Ochoa, Madrid 28049, Spain
关键词
CSFV; E2 gene sequences; molecular epidemiology;
D O I
暂无
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The origin and evolution of the classical swine fever (CSF) epizootic that occurred in Cuba from 1993 to 1997 has been investigated by the analysis of E2 gene sequences from 15 representative viral isolates as well as the vaccine and the challenge strains used in this country. In the phylogenetic tree derived from these sequences, the Cuban isolates were located in a defined cluster within the previously reported genomic subgroup 1.2. This cluster was related, although distinguishable, from the live vaccine used in Cuba since 1965. Two further groups were identified. One of them included the early viruses isolated in the western part of Cuba until 1996 and the strain Margarita, used for vaccine potency tests since 1965. These results are consistent with the strain Margarita being the origin of the western outbreaks. The viruses isolated from 1996 in eastern Cuba defined a related, but independent group. The level of sequence variation observed in this group does not exclude an independent origin for the eastern isolates. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
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页码:61 / 67
页数:7
相关论文
共 20 条
[1]   RECENT ADVANCES IN PESTIVIRUS RESEARCH [J].
COLLETT, MS ;
MOENNIG, V ;
HORZINEK, MC .
JOURNAL OF GENERAL VIROLOGY, 1989, 70 :253-266
[2]  
de Arce HD, 1998, VET RES, V29, P431
[3]  
FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x
[4]   REVERSE-TRANSCRIPTASE PCR ASSAY FOR DETECTION OF HOG-CHOLERA VIRUS [J].
HARDING, M ;
LUTZEWALLACE, C ;
PRUDHOMME, I ;
ZHONG, XH ;
ROLA, J .
JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (10) :2600-2602
[5]  
HIGGINS DG, 1992, COMPUT APPL BIOSCI, V8, P189
[6]  
HOFFMANN MA, 1994, ARCH VIROL, V139, P217
[7]   CLASSICAL SWINE FEVER - GENETIC DETECTION AND ANALYSIS OF DIFFERENCES BETWEEN VIRUS ISOLATES [J].
LOWINGS, JP ;
PATON, DJ ;
SANDS, JJ ;
DEMIA, GM ;
RUTILI, D .
JOURNAL OF GENERAL VIROLOGY, 1994, 75 :3461-3468
[8]   Classical swine fever virus diversity and evolution [J].
Lowings, P ;
Ibata, G ;
Needham, J ;
Paton, D .
JOURNAL OF GENERAL VIROLOGY, 1996, 77 :1311-1321
[9]   MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF THE GENOME OF HOG-CHOLERA VIRUS [J].
MEYERS, G ;
RUMENAPF, T ;
THIEL, HJ .
VIROLOGY, 1989, 171 (02) :555-567
[10]   THE PESTIVIRUSES [J].
MOENNIG, V ;
PLAGEMANN, PGW .
ADVANCES IN VIRUS RESEARCH, 1992, 41 :53-98