Fluorescence Probing Live Single-cell Mass Spectrometry for Direct Analysis of Organelle Metabolism

被引：21
|
作者：
Esaki, Tsuyoshi [1 ]
Masujima, Tsutomu [1 ]
机构：
[1] RIKEN, Quantitat Biol Ctr QBiC, Suita, Osaka 5650874, Japan
来源：
ANALYTICAL SCIENCES | 2015年 / 31卷 / 12期
关键词：
Single-cell analysis; live single-cell mass spectrometry; fluorescence imaging; mitochondria; organelle; METABOLOMICS;
D O I：
10.2116/analsci.31.1211
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Mitochondria in a live HepG2 cell were visualized with a fluorescent probe to specify their location and state in a living cell. Then, mitochondria were selectively captured with a nanospray tip under fluorescence microscope, and thousands of small molecular peaks were revealed and unique steroids specific to mitochondria were also found. This fluorescence imaging combined with live single-cell mass spectrometry opens the door to the analysis of site- and state-specific molecular detection to elucidate precise molecular mechanisms at the single-cell and organelle level.
引用
收藏
页码：1211 / 1213
页数：3
相关论文
共 50 条
  • [1] Fluorescence Probing Live Single-cell Mass Spectrometry for Direct Analysis of Organelle Metabolism
    Tsuyoshi Esaki
    Tsutomu Masujima
    Analytical Sciences, 2015, 31 : 1211 - 1213
  • [2] Live Single-cell Mass Spectrometry for Organelle Metabolomics
    Mizuno, Hajime
    Tsuyama, Naohiro
    Masujima, Tsutomu
    BUNSEKI KAGAKU, 2014, 63 (06) : 477 - 484
  • [3] Live Single-cell Mass Spectrometry
    Tsutomu Masujima
    Analytical Sciences, 2009, 25 : 953 - 960
  • [4] Live Single-cell Mass Spectrometry
    Masujima, Tsutomu
    ANALYTICAL SCIENCES, 2009, 25 (08) : 953 - 960
  • [5] Direct metabolomics for plant cells by live single-cell mass spectrometry
    Fujii, Takashi
    Matsuda, Shuichi
    Tejedor, Monica Lorenzo
    Esaki, Tsuyoshi
    Sakane, Iwao
    Mizuno, Hajime
    Tsuyama, Naohiro
    Masujima, Tsutomu
    NATURE PROTOCOLS, 2015, 10 (09) : 1445 - 1456
  • [6] Direct metabolomics for plant cells by live single-cell mass spectrometry
    Takashi Fujii
    Shuichi Matsuda
    Mónica Lorenzo Tejedor
    Tsuyoshi Esaki
    Iwao Sakane
    Hajime Mizuno
    Naohiro Tsuyama
    Tsutomu Masujima
    Nature Protocols, 2015, 10 : 1445 - 1456
  • [7] Live single-cell molecular analysis by video-mass spectrometry
    Tsuyama, Naohiro
    Mizuno, Hajime
    Tokunaga, Emi
    Masujima, Tsutomu
    ANALYTICAL SCIENCES, 2008, 24 (05) : 559 - 561
  • [8] Live Single-Cell Molecular Analysis by Video-Mass Spectrometry
    Naohiro Tsuyama
    Hajime Mizuno
    Emi Tokunaga
    Tsutomu Masujima
    Analytical Sciences, 2008, 24 : 559 - 561
  • [9] In situ direct live single-cell exhaustive molecular analysis by video-mass spectrometry.
    Mizuno, H.
    Date, S.
    Tsuyama, N.
    Masujima, T.
    MOLECULAR BIOLOGY OF THE CELL, 2012, 23
  • [10] Analysis of the intracellular localization of amiodarone using live single-cell mass spectrometry
    Yahata, Kenji
    Mizuno, Hajime
    Sugiyama, Eiji
    Todoroki, Kenichiro
    JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2021, 205
12345