The use of cell-penetrating peptides as a tool for gene regulation

被引：162

作者：

Järver, P ^{[1
]}

Langel, Ü ^{[1
]}

机构：

[1] Stockholm Univ, Dept Neurochem & Neurotoxicol, SE-10691 Stockholm, Sweden

来源：

DRUG DISCOVERY TODAY | 2004年 / 9卷 / 09期

关键词：

D O I：

10.1016/S1359-6446(04)03042-9

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A novel carrier system that originates from membrane shuttling proteins such as the Drosophila homeobox protein Antennapedia, the HIV-1 transcriptional factor TAT and VP22 from HSV-1 has advantages for targeted delivery compared with standard translocation techniques. This transport system is mediated by so-called cell-penetrating peptides, which consist of short peptide sequences that rapidly translocate large molecules into the cell interior in a seemingly energy- and receptor-independent manner. Cell-penetrating peptides have low toxicity and a high yield of delivery and in the future might become a widely used tool in the field of gene regulation.

引用

页码：395 / 402

页数：8

共 69 条

[1] TAT fusion proteins containing tyrosine 42-deleted IκBα arrest osteoclastogenesis [J].

Abu-Amer, Y ;

Dowdy, SF ;

Ross, FP ;

Clohisy, JC ;

Teitelbaum, SL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (32) :30499-30503

[2] Antisense inhibition of P-glycoprotein expression using peptide-oligonucleotide conjugates [J].

Astriab-Fisher, A ;

Sergueev, DS ;

Fisher, M ;

Shaw, BR ;

Juliano, RL .

BIOCHEMICAL PHARMACOLOGY, 2000, 60 (01) :83-90

[3] A peptide inhibitor of c-Jun N-terminal kinase protects against excitotoxicity and cerebral ischemia [J].

Borsello, T ;

Clarke, PGH ;

Hirt, L ;

Vercelli, A ;

Repici, M ;

Schorderet, DF ;

Bogousslavsky, J ;

Bonny, C .

NATURE MEDICINE, 2003, 9 (09) :1180-1186

[4] A peptide nucleic acid-nuclear localization signal fusion that mediates nuclear transport of DNA [J].

Brandén, LJ ;

Mohamed, AJ ;

Smith, CIE .

NATURE BIOTECHNOLOGY, 1999, 17 (08) :784-787

[5] Designing peptide-based scaffolds as drug delivery vehicles [J].

Brokx, RD ;

Bisland, SK ;

Gariépy, J .

JOURNAL OF CONTROLLED RELEASE, 2002, 78 (1-3) :115-123

[6] THE RETRO-INVERSO FORM OF A HOMEOBOX-DERIVED SHORT PEPTIDE IS RAPIDLY INTERNALIZED BY CULTURED NEURONS - A NEW BASIS FOR AN EFFICIENT INTRACELLULAR DELIVERY SYSTEM [J].

BRUGIDOU, J ;

LEGRAND, C ;

MERY, J ;

RABIE, A .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 214 (02) :685-693

[7] Antennapedia and HIV transactivator of transcription (TAT) "protein transduction domains" promote endocytosis of high molecular weight cargo upon binding to cell surface glycosaminoglycans [J].

Console, S ;

Marty, C ;

García-Echeverría, C ;

Schwendener, R ;

Ballmer-Hofer, K .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (37) :35109-35114

[8] Effects in live cells of a c-myc anti-gene PNA linked to a nuclear localization signal [J].

Cutrona, G ;

Carpaneto, EM ;

Ulivi, M ;

Roncella, S ;

Landt, O ;

Ferrarini, M ;

Boffa, LC .

NATURE BIOTECHNOLOGY, 2000, 18 (03) :300-303

[9]

DEROSSI D, 1994, J BIOL CHEM, V269, P10444

[10] Cellular uptake of Antennapedia Penetratin peptides is a two-step process in which phase transfer precedes a tryptophan-dependent translocation [J].

Dom, G ;

Shaw-Jackson, C ;

Matis, C ;

Bouffioux, O ;

Picard, JJ ;

Prochiantz, A ;

Mingeot-Leclercq, MP ;

Brasseur, R ;

Rezsohazy, R .

NUCLEIC ACIDS RESEARCH, 2003, 31 (02) :556-561

← 1 2 3 4 5 6 7 →