Purification and partial characterization of a novel anti-tumor glycoprotein from cultured mycelia of Grifola frondosa

被引：45
作者：
Cui, Fengjie [1 ,2 ]
Zan, Xinyi [1 ]
Li, Yunhong [1 ]
Yang, Yan [3 ]
Sun, Wenjing [1 ,2 ,4 ]
Zhou, Qiang [2 ,4 ]
Yu, Silian [2 ,4 ]
Dong, Ying [1 ]
机构：
[1] Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China
[2] Jiangxi Prov Engn & Technol Ctr Food Addit Biopro, Dexing 334221, Peoples R China
[3] Shanghai Acad Agr Sci, Natl Engn Res Ctr Edible Fungi, Shanghai 201403, Peoples R China
[4] Parchn Sodium Isovitamin C Co Ltd, Dexing 334221, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2013年 / 62卷
基金：
中国国家自然科学基金; 中国博士后科学基金;
关键词：
Grifola frondosa; Glycoprotein; Purification; Structural characterization; Anti-tumor activity; STRUCTURAL-CHARACTERIZATION; MUSHROOM; LECTIN; POLYSACCHARIDE; APOPTOSIS;
D O I：
10.1016/j.ijbiomac.2013.10.025
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel glycoprotein GFG-3a with the molecular weight of 88.01 kDa and potent anti-tumor activity was isolated from the cultured mycelia of Grifola frondosa GF9801. GFG-3a was heat-sensitive with the decreasing anti-proliferative activity after treated from 56 degrees C to 100 degrees C for 10-120 min. GFG-3a was a glycoprotein with O-glycosylation and contained 6.20% carbohydrate composed of D-arabinose, D-fructose, D-mannose, and D-glucose with a molar ratio of 1.33:4.51:2.46:1.00. FT-IR and NMR spectra proved that GFG-3a contained protein and carbohydrate portions with 3-O-methyl-galactose residues, (1 -> 4)-linked beta-galactose residues, and beta-linked glucose residues. Circular dichroism (CD) revealed that GFG-3a was a predominantly beta-sheet glycoprotein with a relatively small alpha-helical content. Protein sequencing and 3D model of GFG-3a were finally predicted by using MALDI-TOF-MS, NCBI blast search and online SWISS-MODLE Workspace service. Our findings will be a reference for the further structure-activity relationship analysis of the mushroom glycoproteins. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码：684 / 690
页数：7
相关论文
共 27 条
[1]  
Andrea T.B., 1999, P SOC EXP BIOL MED, V221, P281
[2]  
Anja S., 2011, BIORESOURCE TECHNOLO, V102, P9121
[3]  
Butters T.D., 2008, MOL BIOMETHODS HDB, P495
[4]   Isolation, purification and antioxidant activities of polysaccharides from Grifola frondosa [J].
Chen, Gui-tang ;
Ma, Xue-mei ;
Liu, Sheng-tao ;
Liao, Yan-li ;
Zhao, Guo-qiang .
CARBOHYDRATE POLYMERS, 2012, 89 (01) :61-66
[5]   Induction of apoptosis in SGC-7901 cells by polysaccharide-peptide GFPS1b from the cultured mycelia of Grifola frondosa GF9801 [J].
Cui, Feng-Jie ;
Li, Yin ;
Xu, Ying-Ying ;
Liu, Zhi-Qiang ;
Huang, Da-Ming ;
Zhang, Zhi-Cai ;
Tao, Wen-Yi .
TOXICOLOGY IN VITRO, 2007, 21 (03) :417-427
[6]   Optimization of the medium composition for production of mycelial biomass and exo-polymer by Grifola frondosa GF9801 using response surface methodology [J].
Cui, FJ ;
Li, Y ;
Xu, ZH ;
Xu, HY ;
Sun, K ;
Tao, WY .
BIORESOURCE TECHNOLOGY, 2006, 97 (10) :1209-1216
[7]   Structure and immunology of a heteroglycan from somatic hybrid mushroom of Pleurotus florida and Calocybe indica var. APK2 [J].
Dey, Biswajit ;
Bhunia, Sanjoy K. ;
Maity, Kankan K. ;
Patra, Sukesh ;
Mandal, Soumitra ;
Behera, Birendra ;
Maiti, Tapas K. ;
Sikdar, Samir R. ;
Islam, Syed S. .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2013, 52 :312-318
[8]  
He L, 2013, FOOD CHEM, V141, P946, DOI [10.1016/j.foodchem.2013.04.053, 10.1016/j.foodchem.2013.03.044]
[9]   Purification and characterization of a lectin from the mushroom Mycoleptodonoides aitchisonii [J].
Kawagishi, H ;
Takagi, J ;
Taira, T ;
Murata, T ;
Usui, T .
PHYTOCHEMISTRY, 2001, 56 (01) :53-58
[10]   Purification and characterization of a lectin from endophytic fungus Fusarium solani having complex sugar specificity [J].
Khan, Feroz ;
Ahmad, Absar ;
Khan, M. Islam .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2007, 457 (02) :243-251
123