Altered biodistribution of an antibody-enzyme conjugate modified with polyethylene glycol

被引：13
作者：
EnoAmooquaye, EA
Searle, F
Boden, JA
Sharma, SK
Burke, PJ
机构：
[1] Cancer Res. Campaign Laboratories, Department of Medical Oncology, Charing Cross Hospital
来源：
BRITISH JOURNAL OF CANCER | 1996年 / 73卷 / 11期
关键词：
antibody-enzyme; conjugate; polyethylene glycol;
D O I：
10.1038/bjc.1996.253
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Polyethyene glycol modification of the antibody-enzyme conjugate, F(ab')(2)-A5B7-CPG(2), extends its duration in the circulation of nude mice bearing human colonic cancer xenografts (LS174T). Increased concentration of modified conjugate is achieved in the tumour, but residual non-specific enzyme concentrations in normal tissue and blood demonstrate the fundamental requirement to remove or inactivate non-specifically held enzyme in this system.
引用
收藏
页码：1323 / 1327
页数：5
相关论文
共 20 条
  • [1] ABUCHOWSKI A, 1977, J BIOL CHEM, V252, P3578
  • [2] ANTIBODY-DIRECTED ENZYME PRODRUG THERAPY (ADEPT) - REVIEW
    BAGSHAWE, KD
    SHARMA, SK
    SPRINGER, CJ
    ROGERS, GT
    [J]. ANNALS OF ONCOLOGY, 1994, 5 (10) : 879 - 891
  • [3] BERGER H, 1988, BLOOD, V71, P1641
  • [4] PROPERTIES OF 2 URATE OXIDASES MODIFIED BY THE COVALENT ATTACHMENT OF POLY(ETHYLENE GLYCOL)
    CHEN, RHL
    ABUCHOWSKI, A
    VANES, T
    PALCZUK, NC
    DAVIS, FF
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 660 (02) : 293 - 298
  • [5] FRAKER PJ, 1978, BIOCHEM BIOPH RES CO, V80, P849, DOI 10.1016/0006-291X(78)91322-0
  • [6] DETERMINATION OF FREE AMINO GROUPS IN PROTEINS BY TRINITROBENZENESULFONIC ACID
    HABEEB, AFS
    [J]. ANALYTICAL BIOCHEMISTRY, 1966, 14 (03) : 328 - &
  • [7] CHEMICAL MODIFICATION OF RECOMBINANT INTERLEUKIN-2 BY POLYETHYLENE-GLYCOL INCREASES ITS POTENCY IN THE MURINE METH-A SARCOMA MODEL
    KATRE, NV
    KNAUF, MJ
    LAIRD, WJ
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (06) : 1487 - 1491
  • [8] COVALENT LINKAGE OF CARBOXYPEPTIDASE-G2 TO SOLUBLE DEXTRANS .2. INVIVO DISTRIBUTION AND FATE OF CONJUGATES
    MELTON, RG
    WIBLIN, CN
    BASKERVILLE, A
    FOSTER, RL
    SHERWOOD, RF
    [J]. BIOCHEMICAL PHARMACOLOGY, 1987, 36 (01) : 113 - 121
  • [9] OPTIMIZATION OF SMALL-SCALE COUPLING OF A5B7-MONOCLONAL ANTIBODY TO CARBOXYPEPTIDASE-G2
    MELTON, RG
    BOYLE, JMB
    ROGERS, GT
    BURKE, P
    BAGSHAWE, KD
    SHERWOOD, RF
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1993, 158 (01) : 49 - 56
  • [10] THE THERAPEUTIC VALUE OF POLY(ETHYLENE GLYCOL)-MODIFIED PROTEINS
    NUCCI, ML
    SHORR, R
    ABUCHOWSKI, A
    [J]. ADVANCED DRUG DELIVERY REVIEWS, 1991, 6 (02) : 133 - 151
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