DNA Length-dependent Division of a Giant Vesicle-based Model Protocell

被引：28

作者：

Matsuo, Muneyuki ^{[1
,2
]}

Kan, Yumi ^{[3
]}

Kurihara, Kensuke ^{[2
,4
]}

Jimbo, Takehiro ^{[5
]}

Imai, Masayuki ^{[3
,5
]}

Toyota, Taro ^{[1
,6
]}

Hirata, Yuiko ^{[7
]}

Suzuki, Kentaro ^{[7
]}

Sugawara, Tadashi ^{[7
]}

机构：

[1] Univ Tokyo, Grad Sch Arts & Sci, Dept Basic Sci, Meguro Ku, Tokyo 1538902, Japan

[2] Exploratory Res Ctr Life & Living Syst ExCELLS, Dept Creat Res, Okazaki, Aichi 4448787, Japan

[3] Ochanomizu Univ, Grad Sch Sci, Dept Phys, Bunkyo Ku, Tokyo 1128610, Japan

[4] Inst Mol Sci, Biomol Funct, Determent Life & Coordinat Complex Mol Sci, Okazaki, Aichi 4448585, Japan

[5] Tohoku Univ, Grad Sch Sci, Dept Phys, Aoba Ku, Sendai, Miyagi 9808578, Japan

[6] Univ Tokyo, Univ Biol Inst, Bunkyo Ku, Tokyo 1130033, Japan

[7] Kanagawa Univ, Fac Sci, Dept Chem, Hiratsuka, Kanagawa 2591293, Japan

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

关键词：

PHOSPHOLIPID-MEMBRANES; SELF-REPRODUCTION; RNA; DYNAMICS; POLYMER;

D O I：

10.1038/s41598-019-43367-4

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

DNA is an essential carrier of sequence-based genetic information for all life today. However, the chemical and physical properties of DNA may also affect the structure and dynamics of a vesicle-based model protocell in which it is encapsulated. To test these effects, we constructed a polyethylene glycol-grafted giant vesicle system capable of undergoing growth and division. The system incorporates a specific interaction between DNA and lipophilic catalysts as well as components of PCR. We found that vesicle division depends on the length of the encapsulated DNA, and the self-assembly of an internal supramolecular catalyst possibly leads to the direct causal relationship between DNA length and the capacity of the vesicle to self-reproduce. These results may help elucidate how nucleic acids could have functioned in the division of prebiotic protocells.

引用

页数：11

共 2 条

[1] Monitoring mRNA and Protein Levels in Bulk and in Model Vesicle-Based Artificial Cells
van Nies, Pauline
Canton, Alicia Soler
Nourian, Zohreh
Danelon, Christophe
RIBOSWITCHES AS TARGETS AND TOOLS, 2015, 550 : 187 - 214
[2] Length-Dependent Melting Kinetics of Short DNA Oligonucleotides Using Temperature-Jump IR Spectroscopy
Menssen, Ryan J.
Tokmakoff, Andrei
JOURNAL OF PHYSICAL CHEMISTRY B, 2019, 123 (04) : 756 - 767

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