Norbornene Probes for the Detection of Cysteine Sulfenic Acid in Cells

被引：38

作者：

Alcock, Lisa J. ^{[1
,2
]}

Oliveira, Bruno L. ^{[3
]}

Deery, Michael J. ^{[4
]}

Pukala, Tara L. ^{[5
]}

Perkins, Michael V. ^{[1
]}

Bernardes, Goncalo J. L. ^{[3
,6
]}

Chalker, Justin M. ^{[1
,2
]}

机构：

[1] Flinders Univ S Australia, Coll Sci & Engn, Sturt Rd, Bedford Pk, SA 5042, Australia

[2] Flinders Univ S Australia, Inst NanoScale Sci & Technol, Sturt Rd, Bedford Pk, SA 5042, Australia

[3] Univ Cambridge, Dept Chem, Lensfield Rd, Cambridge CB2 1EW, England

[4] Univ Cambridge, Dept Biochem, Cambridge Syst Biol Ctr, Cambridge Ctr Prote, Tennis Court Rd, Cambridge CB2 1QW, England

[5] Univ Adelaide, Sch Phys Sci, Adelaide, SA 5005, Australia

[6] Univ Lisbon, Fac Med, Inst Mol Med, Ave Prof Egas Moniz, P-1649028 Lisbon, Portugal

来源：

ACS CHEMICAL BIOLOGY | 2019年 / 14卷 / 04期

基金：

欧洲研究理事会; 英国工程与自然科学研究理事会; 澳大利亚研究理事会;

关键词：

CHEMICAL PROBES; DIMEDONE; REACTIVITY;

D O I：

10.1021/acschembio.8b01104

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Norbornene derivatives were validated as probes for cysteine sulfenic acid on proteins and in live cells. Trapping sulfenic acids with norbornene probes is highly selective and revealed a different reactivity profile than the traditional dimedone reagent. The norbornene probe also revealed a superior chemoselectivity when compared to a commonly used dimedone probe. Together, these results advance the study of cysteine oxidation in biological systems.

引用

页码：594 / 598

页数：5

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