Novel female-specific trans-spliced and alternative splice forms of dsx in the silkworm Bombyx mori

被引：16

作者：

Duan, Jianping ^{[1
]}

Xu, Hanfu ^{[1
]}

Wang, Feng ^{[1
]}

Ma, Sanyuan ^{[1
]}

Zha, Xingfu ^{[1
]}

Guo, Huizhen ^{[1
]}

Zhao, Ping ^{[1
]}

Xia, Qingyou ^{[1
]}

机构：

[1] Southwest Univ, State Key Lab Silkworm Genome Biol, Chongqing 400715, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2013年 / 431卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Doublesex; Sexual development; Trans-splicing; Bombyx mori; SEX-DETERMINATION; DOUBLESEX GENE; DROSOPHILA-MELANOGASTER; DOMESTICATED SILKWORM; DIFFERENTIATION; EXPRESSION; DMRT1; MOUSE; IDENTIFICATION; CHROMOSOME;

D O I：

10.1016/j.bbrc.2012.12.062

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Bombyx mori doublesex gene (Bmdsx) plays an important role in somatic sexual development. Its premRNA splices in a sex-specific manner to generate two female-specific and one male-specific splice forms. The present study investigated six novel dsx variants generated by trans-splicing between female dsx transcripts and two additional novel genes, dsrl and dsr2. Expression analysis indicated that Bmdsx-dsr1 represented splicing noise, whereas dsr2, which trans-spliced with dsx to generate five variants, regulated the expression of the female-specific B. mori dsx transcript Bmdsx(F)s. We unexpectedly found a novel exon 2n insertion during Bmdsx transcription, which did not influence the validity of the novel protein, BmDSX(F3) Ectopic expression of BmDSX(F3) repressed the pheromone-binding protein gene and the testis-specific gene A2 in males, and activated of the storage protein 1 gene. Our findings suggest that trans-splicing is a novel regulatory function of Bmdsx, which participates in female sexual development by regulating the expression of three BmDSX(F) proteins. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：630 / 635

页数：6

共 42 条

[31] Role of the male BmDSX protein in the sexual differentiation of Bombyx mori [J].

Suzuki, MG ;

Funaguma, S ;

Kanda, T ;

Tamura, T ;

Shimada, T .

EVOLUTION & DEVELOPMENT, 2005, 7 (01) :58-68

[32] Analysis of the biological functions of a doublesex homologue in Bombyx mori [J].

Suzuki, MG ;

Funaguma, S ;

Kanda, T ;

Tamura, T ;

Shimada, T .

DEVELOPMENT GENES AND EVOLUTION, 2003, 213 (07) :345-354

[33] The mechanism of sex-specific splicing at the doublesex gene is different between Drosophila melanogaster and Bombyx mori [J].

Suzuki, MG ;

Ohbayashi, F ;

Mita, K ;

Shimada, T .

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2001, 31 (12) :1201-1211

[34] Germline transformation of the silkworm Bombyx mori L-using a piggyBac transposon-derived vector [J].

Tamura, T ;

Thilbert, C ;

Royer, C ;

Kanda, T ;

Abraham, E ;

Kamba, M ;

Komoto, N ;

Thomas, JL ;

Mauchamp, B ;

Chavancy, G ;

Shirk, P ;

Fraser, M ;

Prudhomme, JC ;

Couble, P .

NATURE BIOTECHNOLOGY, 2000, 18 (01) :81-84

[35] Evolution of Sex-Specific Traits through Changes in HOX-Dependent doublesex Expression [J].

Tanaka, Kohtaro ;

Barmina, Olga ;

Sanders, Laura E. ;

Arbeitman, Michelle N. ;

Kopp, Artyom .

PLOS BIOLOGY, 2011, 9 (08)

[36] Reference genes identified in the silkworm Bombyx mori during metamorphism based on oligonucleotide microarray and confirmed by qRT-PCR [J].

Wang, Gen-Hong ;

Xia, Qing-You ;

Cheng, Dao-Jun ;

Duan, Jun ;

Zhao, Ping ;

Chen, Jie ;

Zhu, Li .

INSECT SCIENCE, 2008, 15 (05) :405-413

[37] Sexually dimorphic regulation of the Wingless morphogen controls sex-specific segment number in Drosophila [J].

Wang, Wei ;

Kidd, Bryan J. ;

Carroll, Sean B. ;

Yoder, John H. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (27) :11139-11144

[38]

Wang Z., 2009, MOL BIOL REP

[39] Microarray-based gene expression profiles in multiple tissues of the domesticated silkworm, Bombyx mori [J].

Xia, Qingyou ;

Cheng, Daojun ;

Duan, Jun ;

Wang, Genhong ;

Cheng, Tingcai ;

Zha, Xingfu ;

Liu, Chun ;

Zhao, Ping ;

Dai, Fangyin ;

Zhang, Ze ;

He, Ningjia ;

Zhang, Liang ;

Xiang, Zhonghuai .

GENOME BIOLOGY, 2007, 8 (08)

[40] A draft sequence for the genome of the domesticated silkworm (Bombyx mori) [J].

Xia, QY ;

Zhou, ZY ;

Lu, C ;

Cheng, DJ ;

Dai, FY ;

Li, B ;

Zhao, P ;

Zha, XF ;

Cheng, TC ;

Chai, CL ;

Pan, GQ ;

Xu, JS ;

Liu, C ;

Lin, Y ;

Qian, JF ;

Hou, Y ;

Wu, ZL ;

Li, GR ;

Pan, MH ;

Li, CF ;

Shen, YH ;

Lan, XQ ;

Yuan, LW ;

Li, T ;

Xu, HF ;

Yang, GW ;

Wan, YJ ;

Zhu, Y ;

Yu, MD ;

Shen, WD ;

Wu, DY ;

Xiang, ZH ;

Yu, J ;

Wang, J ;

Li, RQ ;

Shi, JP ;

Li, H ;

Li, GY ;

Su, JN ;

Wang, XL ;

Li, GQ ;

Zhang, ZJ ;

Wu, QF ;

Li, J ;

Zhang, QP ;

Wei, N ;

Xu, JZ ;

Sun, HB ;

Dong, L ;

Liu, DY .

SCIENCE, 2004, 306 (5703) :1937-1940

← 1 2 3 4 5 →